Nanosecond Pulsed Electric Field Inhibits Cancer Growth Followed by Alteration in Expressions of NF-κB and Wnt/β-Catenin Signaling Molecules
Open Access
- 17 September 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (9), e74322
- https://doi.org/10.1371/journal.pone.0074322
Abstract
Cancer remains a leading cause of death worldwide and total number of cases globally is increasing. Novel treatment strategies are therefore desperately required for radical treatment of cancers and long survival of patients. A new technology using high pulsed electric field has emerged from military application into biology and medicine by applying nsPEF as a means to inhibit cancer. However, molecular mechanisms of nsPEF on tumors or cancers are still unclear. In this paper, we found that nsPEF had extensive biological effects in cancers, and clarified its possible molecular mechanisms in vitro and in vivo. It could not only induce cell apoptosis via dependent-mitochondria intrinsic apoptosis pathway that was triggered by imbalance of anti- or pro-apoptosis Bcl-2 family proteins, but also inhibit cell proliferation through repressing NF-κB signaling pathway to reduce expressions of cyclin proteins. Moreover, nsPEF could also inactivate metastasis and invasion in cancer cells by suppressing Wnt/β-Catenin signaling pathway to down-regulating expressions of VEGF and MMPs family proteins. More importantly, nsPEF could function safely and effectively as an anti-cancer therapy through inducing tumor cell apoptosis, destroying tumor microenvironment, and depressing angiogenesis in tumor tissue in vivo. These findings may provide a creative and effective therapeutic strategy for cancers.Keywords
This publication has 50 references indexed in Scilit:
- Wnt/β-Catenin Signaling and DiseaseCell, 2012
- Regulation of apoptosis by the circadian clock through NF-κB signalingProceedings of the National Academy of Sciences of the United States of America, 2011
- Hallmarks of Cancer: The Next GenerationCell, 2011
- An apoptosis targeted stimulus with nanosecond pulsed electric fields (nsPEFs) in E4 squamous cell carcinomaApoptosis, 2011
- Optimized nanosecond pulsed electric field therapy can cause murine malignant melanomas to self‐destruct with a single treatmentInternational Journal of Cancer, 2010
- Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008International Journal of Cancer, 2010
- NF-κB and cancer—identifying targets and mechanismsCurrent Opinion in Genetics & Development, 2008
- Nuclear IKK activity leads to dysregulated Notch-dependent gene expression in colorectal cancerProceedings of the National Academy of Sciences of the United States of America, 2007
- The Hallmarks of CancerCell, 2000
- Activators and target genes of Rel/NF-κB transcription factorsOncogene, 1999