Cryo-EM structure of a 3D DNA-origami object
- 19 November 2012
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 109 (49), 20012-20017
- https://doi.org/10.1073/pnas.1215713109
Abstract
A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback.Keywords
This publication has 36 references indexed in Scilit:
- Prevention of overfitting in cryo-EM structure determinationNature Methods, 2012
- A Bayesian View on Cryo-EM Structure DeterminationJournal of Molecular Biology, 2012
- Multilayer DNA Origami Packed on Hexagonal and Hybrid LatticesJournal of the American Chemical Society, 2011
- Tilt-Pair Analysis of Images from a Range of Different Specimens in Single-Particle Electron CryomicroscopyJournal of Molecular Biology, 2011
- Multilayer DNA Origami Packed on a Square LatticeJournal of the American Chemical Society, 2009
- From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystalNature, 2009
- Self-assembly of DNA into nanoscale three-dimensional shapesNature, 2009
- Image processing for electron microscopy single-particle analysis using XMIPPNature Protocols, 2008
- Flexible Fitting of Atomic Structures into Electron Microscopy Maps Using Molecular DynamicsStructure, 2008
- EMAN: Semiautomated Software for High-Resolution Single-Particle ReconstructionsJournal of Structural Biology, 1999