Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii
- 15 February 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 218 (1), 113-118
- https://doi.org/10.1042/bj2180113
Abstract
Glyoxylate dehydrogenase (glyoxylate: NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.This publication has 9 references indexed in Scilit:
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