Angular reconstitution-based 3D reconstructions of nanomolecular structures from superresolution light-microscopy images
Open Access
- 15 August 2017
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 114 (35), 9273-9278
- https://doi.org/10.1073/pnas.1704908114
Abstract
Superresolution light microscopy allows the imaging of labeled supramolecular assemblies at a resolution surpassing the classical diffraction limit. A serious limitation of the superresolution approach is sample heterogeneity and the stochastic character of the labeling procedure. To increase the reproducibility and the resolution of the superresolution results, we apply multivariate statistical analysis methods and 3D reconstruction approaches originally developed for cryogenic electron microscopy of single particles. These methods allow for the reference-free 3D reconstruction of nanomolecular structures from two-dimensional superresolution projection images. Since these 2D projection images all show the structure in high-resolution directions of the optical microscope, the resulting 3D reconstructions have the best possible isotropic resolution in all directions.Keywords
This publication has 45 references indexed in Scilit:
- Single-Molecule Localization Microscopy in EukaryotesChemical Reviews, 2017
- Advances in the field of single-particle cryo-electron microscopy over the last decadeNature Protocols, 2017
- The cryo-EM structure of a ribosome–Ski2-Ski3-Ski8 helicase complexScience, 2016
- Structure of a type IV secretion systemNature, 2014
- Distribution of ESCRT Machinery at HIV Assembly Sites Reveals Virus Scaffolding of ESCRT SubunitsScience, 2014
- Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle AveragingScience, 2013
- Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organizationNature, 2012
- A guide to super-resolution fluorescence microscopyThe Journal of cell biology, 2010
- Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructureProceedings of the National Academy of Sciences of the United States of America, 2009
- Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination MicroscopyScience, 2008