Brownian modulated optical nanoprobes
- 30 December 2003
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 84 (1), 154-156
- https://doi.org/10.1063/1.1637963
Abstract
Brownian modulated optical nanoprobes (Brownian MOONs) are fluorescent micro- and nanoparticles that resemble moons: one hemisphere emits a bright fluorescent signal, while an opaque metal darkens the other hemisphere. Brownian motion causes the particles to tumble and blink erratically as they rotate literally through the phases of the moon. The fluctuating probe signals are separated from optical and electronic backgrounds using principal components analysis or images analysis. Brownian MOONs enable microrheological measurements on size scales and timescales that are difficult to study with other methods. Local chemical concentrations can be measured simultaneously, using spectral characteristics of indicator dyes embedded within the MOONs.Keywords
This publication has 17 references indexed in Scilit:
- Magnetically modulated optical nanoprobesApplied Physics Letters, 2003
- Ultrastructural Changes Accompanying the Mechanical Deformation of Bone Tissue: A Raman Imaging StudyCalcified Tissue International, 2003
- Force and torque measurements using magnetic micro beads for single molecule biophysicsOptics Communications, 2002
- Ultrafine Particles Cause Cytoskeletal Dysfunctions in MacrophagesToxicology and Applied Pharmacology, 2002
- MicrorheologyCurrent Opinion in Colloid & Interface Science, 1999
- Tracer microrheology in complex fluidsCurrent Opinion in Colloid & Interface Science, 1998
- Direct Measurement of the Torsional Rigidity of Single Actin FilamentsJournal of Molecular Biology, 1996
- Mechanism and size cutoff for steric exclusion from actin-rich cytoplasmic domainsBiophysical Journal, 1996
- Magnetic particle motions within living cells. Measurement of cytoplasmic viscosity and motile activityBiophysical Journal, 1987
- Magnetic particle motions within living cells. Physical theory and techniquesBiophysical Journal, 1987