Distribution of genetic diversity in wild European populations of prickly lettuce (Lactuca serriola): implications for plant genetic resources management
- 25 May 2010
- journal article
- research article
- Published by Cambridge University Press (CUP) in Plant Genetic Resources: Characterization and Utilization
- Vol. 8 (2), 171-181
- https://doi.org/10.1017/s1479262110000134
Abstract
Genetic variation inLactuca serriola, the closest wild relative of cultivated lettuce, was studied across Europe from the Czech Republic to the United Kingdom, using three molecular marker systems, simple sequence repeat (SSR, microsatellites), AFLP and nucleotide-binding site (NBS) profiling. The ‘functional’ marker system NBS profiling, targeting disease resistance genes of the NBS/LRR family, did not show marked differences in genetic diversity parameters to the other systems. The autogamy of the species resulted in low observed heterozygosity and high population differentiation. Intra-population variation ranged from complete homogeneity to nearly complete heterogeneity. The highest genetic diversity was found in central Europe. The SSR results were compared to SSR variation screened earlier in the lettuce collection of the Centre for Genetic Resources, the Netherlands (CGN). In the UK, practically only a single SSR genotype was found. This genotype together with a few other common SSR genotypes comprised a large part of the plants sampled on the continent. Among the ten most frequent SSR genotypes observed, eight were already present in the CGN collection. Overall, the CGN collection appears to already have a fair representation of genetic variation from NW Europe. The results are discussed in relation to sampling strategies for improving genebank collections of crop wild relatives.Keywords
This publication has 26 references indexed in Scilit:
- Comparison of anonymous and targeted molecular markers for the estimation of genetic diversity in ex situ conserved LactucaTheoretical and Applied Genetics, 2009
- The utility of NBS profiling for plant systematics: a first study in tuber-bearing Solanum speciesÖsterreichische botanische Zeitschrift, 2008
- Analysis of wild Lactuca accessions: conservation and identification of redundancyPlant Genetic Resources: Characterization and Utilization, 2008
- Evolution and Genetic Population Structure of Prickly Lettuce (Lactuca serriola) and Its RGC2 Resistance Gene ClusterGenetics, 2008
- Almost Forgotten or Latest Practice? AFLP applications, analyses and advancesTrends in Plant Science, 2007
- Invasive behaviour of Lactuca serriola (Asteraceae) in the Netherlands: Spatial distribution and ecological amplitudeBasic and Applied Ecology, 2006
- Nucleotide-binding site (NBS) profiling of genetic diversity in durum wheatGenome, 2006
- Microsatellite retrieval in lettuce (Lactuca sativa L.)Genome, 1999
- Microsatellite retrieval in lettuce (Lactuca sativa L.)Genome, 1999
- Analysis of Gene Diversity in Subdivided PopulationsProceedings of the National Academy of Sciences, 1973