Molecularly Imprinted Sol−Gel Nanotubes Membrane for Biochemical Separations

Abstract

In this study, we report a simple procedure for applying molecular imprinting functional groups to the inner surfaces of the template-synthesized sol−gel nanotubes for chemical separation of estrone. The silica nanotubes were synthesized within the pores of nanopore alumina template membranes using a sol−gel method by simultaneous hydrolysis of a silica monomer−imprinted molecule complex and tetraethoxysilane (TEOS). A covalent imprinting strategy was employed by generating a sacrificial spacer through the reaction of the isocyanate group of 3-(triethoxysilyl)propyl isocyanate and a phenol moiety of estrone to form a thermally cleavable urethane bond. This allowed us to remove the imprinted estrone by simple thermal reaction and to simultaneously introduce functional groups into the cavity formed by the silica nanotubes. Experiments indicated that estrone could be bound selectively by such an approach and have a binding affinity of 864 ± 137 (n = 3).