Detection of Epidermal Growth Factor Receptors and E‐Cadherins in the Basolateral Membrane of A431 Cells by Laser Scanning Fluorescence Microscopy
Open Access
- 1 January 1991
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 82 (1), 8-11
- https://doi.org/10.1111/j.1349-7006.1991.tb01737.x
Abstract
We have examined the localization of epidermal growth factor (EGF) receptors and E‐cadherins in cultured A431 cells, an epidermoid carcinoma cell line, using a laser scanning fluorescence microscope. The fluorescence signals generated by monoclonal antihodies against EGF receptor and E‐cadherin were localized mainly in the cell‐cell contact sites, about 3 μm in width. When these areas for cell adhesion were scanned perpendicularly, fluorescence was detected from a point 4μ from the cell base for a distance of 8 μm towards the cell surface (about 16 μm from the cell base). These data suggest the subcellular localization of EGF receptors and E‐cadherins in the basolateral membrane of A431 cells.Keywords
This publication has 17 references indexed in Scilit:
- Cell-cell adhesion mediated by binding of membrane-anchored transforming growth factor alpha to epidermal growth factor receptors promotes cell proliferation.Proceedings of the National Academy of Sciences, 1990
- Distribution of EGF receptor expressing and DNA replicating epidermal cells in psoriasis vulgaris and Bowen's diseaseBritish Journal of Dermatology, 1988
- Identification and Characterization of Insulin Receptors in Basolateral Membranes of Dog Intestinal MucosaDiabetes, 1987
- An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.The Journal of cell biology, 1987
- Monoclonal antibody that immunoreacts with a subclass of human receptors for epidermal growth factor.Cell Structure and Function, 1985
- Comparison of Epidermal Growth Factor Binding and Receptor Distribution in Normal Human Epidermis and Epidermal AppendagesJournal of Investigative Dermatology, 1984
- Cell Genetic Analysis of the Receptor Systems for Bioactive PolypeptidesPublished by Springer Science and Business Media LLC ,1984
- Epidermal growth factor receptorsMolecular and Cellular Biochemistry, 1981
- Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431.Proceedings of the National Academy of Sciences, 1978