Effects of zinc bacitracin, bird age and access to range on bacterial microbiota in the ileum and caeca of broiler chickens
Open Access
- 14 January 2008
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 104 (5), 1372-1382
- https://doi.org/10.1111/j.1365-2672.2007.03699.x
Abstract
Aims: Determining the effects of zinc bacitracin, bird age and access to range on bacterial microbiota in the ileum and caeca of broilers. Methods and Results: 16S rRNA gene‐based polymerase chain reaction‐based denaturing gradient gel electrophoresis (PCR–DGGE) profiling, DNA sequencing and real‐time quantitative PCR techniques were used. The richness of both ileal and caecal microbiota increased with chicken age. The microbiota from those birds of the same age demonstrated relatively similar PCR–DGGE profiles and tended to form closely related clusters in the relatedness analyses. Dietary treatment with bacitracin (50 mg kg−1) and access to range did not change the richness but altered the composition of the microbiota. The impact of bacitracin was particularly obvious in 3‐day‐old chicks. Lactobacilli were abundant in the caecal microbiota of 3‐day‐old chicks regardless of the dietary treatment with bacitracin. The access to range enriched Bifidobacterium in both the ileum and caeca. Conclusions: Bacitracin, bird age and access to range all influenced bacterial microbiota in the ileum and caeca of broilers, with bird age having the greatest apparent effect. Significance and Impact of the Study: Providing useful information for the development of antibiotic replacement therapy for poultry production.Keywords
This publication has 29 references indexed in Scilit:
- 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to cecaFEMS Microbiology Ecology, 2007
- Characterization of Intestinal Microbiota and Response to Dietary Virginiamycin Supplementation in the Broiler ChickenApplied and Environmental Microbiology, 2006
- Effects of Orally Administered Tetracycline on the Intestinal Community Structure of Chickens and on tet Determinant Carriage by Commensal Bacteria and Campylobacter jejuniApplied and Environmental Microbiology, 2005
- The gene encoding xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp) is conserved amongBifidobacteriumspecies within a more variable region of the genome and both are useful for strain identificationFEMS Microbiology Letters, 2005
- Detection and Identification of Lactobacillus Species in Crops of Broilers of Different Ages by Using PCR-Denaturing Gradient Gel Electrophoresis and Amplified Ribosomal DNA Restriction AnalysisApplied and Environmental Microbiology, 2003
- Molecular analysis of bacterial populations in the ileum of broiler chickens and comparison with bacteria in the cecumFEMS Microbiology Ecology, 2002
- 16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler ChickensApplied and Environmental Microbiology, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Percent G+C Profiling Accurately Reveals Diet-Related Differences in the Gastrointestinal Microbial Community of Broiler ChickensApplied and Environmental Microbiology, 2001
- Phylogenetic Analysis of Rumen Bacteria by Comparative Sequence Analysis of Cloned 16S rRNA GenesßAnaerobe, 1998