The evaluation of a nucleoprotein ELISA for the detection of equine influenza antibodies and the differentiation of infected from vaccinated horses (DIVA)
Open Access
- 14 November 2013
- journal article
- Published by Wiley in Influenza and Other Respiratory Viruses
- Vol. 7 (s4), 73-80
- https://doi.org/10.1111/irv.12195
Abstract
Antibodies against equine influenza virus (EIV) are traditionally quantified by haemagglutination inhibition (HI) or single radial haemolysis (SRH). To evaluate an ELISA for the detection of antibodies against influenza nucleoprotein in the diagnosis and surveillance of equine influenza (EI). The ELISA was compared with the SRH and HI tests. Serial serum samples from 203 naturally and 14 experimentally infected horses, from 60 weanlings following primary vaccination with five different vaccines (two whole inactivated vaccines, two ISCOM-based subunit vaccines and a recombinant canarypox virus vaccine) and from 44 adult horses following annual booster vaccination with six different vaccines were analysed. Fewer seroconversions were detected in clinical samples by ELISA than by SRH or HI but ELISA was more sensitive than SRH in naïve foals post-experimental infection. The ELISA did not detect the antibody response to vaccination with the recombinant canarypox virus vaccine confirming the usefulness of the combination of this kit and vaccine to differentiate between naturally infected and vaccinated horses, that is, DIVA. No DIVA capacity was evident with the other vaccines. The results suggest that this ELISA is a useful supplementary test for the diagnosis of EI although less sensitive than HI or SRH. It is an appropriate test for EI surveillance in a naïve population and may be combined with the recombinant canarypox virus vaccine but not with other commercially available subunit vaccines, in a DIVA strategy.Keywords
This publication has 21 references indexed in Scilit:
- Whole inactivated equine influenza vaccine: Efficacy against a representative clade 2 equine influenza virus, IFNgamma synthesis and duration of humoral immunityVeterinary Microbiology, 2013
- Accelerated vaccination schedule provides protective levels of antibody and complete herd immunity to equine influenzaEquine Veterinary Journal, 2012
- ISCOM-matrix-based equine influenza (EIV) vaccine stimulates cell-mediated immunity in the horseVeterinary Immunology and Immunopathology, 2012
- A comparison of antibody responses to commercial equine influenza vaccines following primary vaccination of Thoroughbred weanlings—A randomised blind studyVaccine, 2011
- Evaluating the effectiveness of the response to equine influenza in the Australian outbreak and the potential role of early vaccinationAustralian Veterinary Journal, 2011
- A comparative antibody study of the potential susceptibility of Thoroughbred and non‐Thoroughbred horse populations in Ireland to equine influenza virusInfluenza and Other Respiratory Viruses, 2010
- Efficacy of a whole inactivated EI vaccine against a recent EIV outbreak isolate and comparative detection of virus sheddingVeterinary Immunology and Immunopathology, 2010
- Vaccination against equine influenza: Quid novi?Vaccine, 2006
- Field Studies on Equine Influenza Vaccination Regimes in Thoroughbred Foals and YearlingsThe Veterinary Journal, 2001
- Duration of protective efficacy of equine influenza immunostimulating complex/tetanus vaccinesPublished by Wiley ,1994