Epstein-Barr Virus-Induced Changes in B-Lymphocyte Gene Expression
Open Access
- 15 October 2002
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (20), 10427-10436
- https://doi.org/10.1128/jvi.76.20.10427-10436.2002
Abstract
To elucidate the mechanisms by which Epstein-Barr virus (EBV) latency III gene expression transforms primary B lymphocytes to lymphoblastoid cell lines (LCLs), the associated alterations in cell gene expression were assessed by using 4,146 cellular cDNAs arrayed on nitrocellulose filters and real-time reverse transcription-PCR (RT-PCR). A total of 1,405 of the 4,146 cDNAs were detected using cDNA probes from poly(A)+RNA of IB4 LCLs, a non-EBV-infected Burkitt's lymphoma (BL) cell line, BL41, or EBV latency III-converted BL41 cells (BL41EBV). Thirty-eight RNAs were consistently twofold more abundant in the IB4 LCL and BL41EBV than in BL41 by microarray analysis. Ten of these are known to be EBV induced. A total of 23 of 28 newly identified EBV-induced genes were confirmed by real-time RT-PCR. In addition, nine newly identified genes and CD10 were EBV repressed. These EBV-regulated genes encode proteins involved in signal transduction, transcription, protein biosynthesis and degradation, and cell motility, shape, or adhesion. Seven of seven newly identified EBV-induced RNAs were more abundant in newly established LCLs than in resting B lymphocytes. Surveys of eight promoters of newly identified genes implicate NF-κB or PU.1 as potentially important mediators of EBV-induced effects through LMP1 or EBNA2, respectively. Thus, examination of the transcriptional effects of EBV infection can elucidate the molecular mechanisms by which EBV latency III alters B lymphocytes.Keywords
This publication has 178 references indexed in Scilit:
- Novel NEMO/IκB Kinase and NF-κB Target Genes at the Pre-B to Immature B Cell TransitionPublished by Elsevier BV ,2001
- Distinct types of diffuse large B-cell lymphoma identified by gene expression profilingNature, 2000
- The Transcriptional Program in the Response of Human Fibroblasts to SerumScience, 1999
- Genomic Organization of Two Novel Genes on Human Xq28: Compact Head to Head Arrangement ofIDHγ andTRAPδ Is Conserved in Rat and MouseGenomics, 1997
- Cloning and Characterization of a Novel Human DNaseBiochemical and Biophysical Research Communications, 1997
- The Structure of the Human Intron-Containing S8 Ribosomal Protein Gene and Determination of its Chromosomal Location at 1p32-p34.1Genomics, 1993
- The cystatins: Protein inhibitors of cysteine proteinasesFEBS Letters, 1991
- Isolation of a normal B cell subset with a Burkitt‐like phenotype and transformation in vitro with Epstein‐Barr virusInternational Journal of Cancer, 1988
- EBV‐negative and ‐positive burkitt cell lines variably express receptors for B‐cell activation and differentiationInternational Journal of Cancer, 1986
- Secretion of γ‐Interferon at the Cellular LevelScandinavian Journal of Immunology, 1984