A novel dehydroascorbate reductase from spinach chloroplasts homologous to plant trypsin inhibitor
- 26 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (2), 159-162
- https://doi.org/10.1016/0014-5793(94)00947-3
Abstract
Dehydroascorbate reductase has been isolated from spinach chloroplasts and purified to apparent homogeneity. The N-terminal amino acid sequence of the enzyme is homologous to the Kunitz-type trypsin inhibitors from plant sources. It is shown that spinach DHA reductase and soybean trypsin inhibitor are both capable of reducing dehydroascorbate when in the reduced (thiol) form but acquire trypsin-inhibiting activity in the oxidized (disulfide) state. Reduced chloroplast thioredoxins also reduce dehydroascorbate.This publication has 15 references indexed in Scilit:
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