Isolation of Mitochondria from the CNS
Open Access
- 1 July 2010
- journal article
- unit
- Published by Wiley in Current Protocols in Neuroscience
- Vol. 52 (1), 7.22.1-7.22.12
- https://doi.org/10.1002/0471142301.ns0722s52
Abstract
This unit contains a protocol describing the isolation of brain mitochondria by using discontinuous Percoll gradient centrifugation. The Percoll density gradient centrifugation separates synaptosomes, myelin, and free nonsynaptic mitochondria released from cells during tissue homogenization into individual fractions. Mitochondria entrapped in synaptosomes (synaptic mitochondria) can be liberated using nitrogen cavitation and then further purified by Percoll gradient centrifugation. These methods yield mitochondria that exhibit good respiratory coupling and high respiratory rates. Curr. Protoc. Neurosci. 52:7.22.1‐7.22.12. © 2010 by John Wiley & Sons, Inc.Keywords
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