Anin vitrosystem for testing leucocyte and leukaemic cell line adhesion to synthetic fibres
- 20 December 2001
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 115 (3), 664-671
- https://doi.org/10.1046/j.1365-2141.2001.03137.x
Abstract
Leucocyte adhesion is an important phenomenon in antimicrobial defence, inflammation and immunological mechanisms and has been shown to be dependent upon specialized adhesion molecules. To prevent side-effects related to blood transfusion (e.g. anti-human leucocyte antigen immunization and transmission of infectious agents) leucocyte reduction of blood products is now systematically performed in various countries. The most common system used for leucoreduction is blood filtration. For further understanding of the mechanisms responsible for the interaction between leucocytes and the fibres present in filters we used a flow chamber to study the adhesion of leucocytes and leukaemic cell lines to different types of fibre. Adhesion was quantified using video-microscopy and computer image analysis. Our results demonstrate that adhesion to filter fibres was dependent on the expression of β2-integrins CD11–-CD18 and was inhibited by anti-CD18. The amount of fibres present, their spatial arrangement and the physicochemical characteristics of the fibres were important factors in leucocyte adhesion. Leucocyte adhesion was the highest to polyethylene terephthalate (PET) and polyimide fibres. Lymphocytes or lymphocytic cell lines were poorly adherent to PET fibres. The retaining capacity of leucocyte filters can be improved by taking into account the different parameters for the design of new filtersKeywords
This publication has 24 references indexed in Scilit:
- Human promyelocytic cell line: a convenient tool for studying the molecular basis of WBC filtrationTransfusion, 2000
- Antibodies against Mac-1 Attenuate Neutrophil Accumulation after Traumatic Brain Injury in RatsJournal of Neurotrauma, 1996
- Poly(ethyleneimine) modified filters for the removal of leukocytes from bloodJournal of Biomedical Materials Research, 1993
- Leukodepletion Blood Filters: Filter Design and Mechanisms of Leukocyte RemovalTransfusion Medicine Reviews, 1993
- The mechanism of white cell reduction by synthetic fiber cell filtersTransfusion, 1993
- A Simplified Method for Culture of Endothelial Cells and Analysis of Adhesion of Blood Cells under Conditions of FlowMicrovascular Research, 1993
- In vitro leucocyte adhesion to modified polyurethane surfaces: I. Effect of ionizable functional groupsBiomaterials, 1992
- Mechanical Properties of HL60 Cells: Role of Stimulation and Differentiation in Retention in Capillary-sized PoresAmerican Journal of Respiratory Cell and Molecular Biology, 1991
- Kinetics of cell adhesion to polymer surfacesJournal of Biomedical Materials Research, 1988
- FEBRILE TRANSFUSION REACTIONS CAUSED BY SENSITIVITY TO DONOR LEUKOCYTES AND PLATELETSJournal of the American Medical Association, 1957