Characterization of Chromosomal Regions Conserved in Yersinia pseudotuberculosis and Lost by Yersinia pestis
- 1 October 2008
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 76 (10), 4592-4599
- https://doi.org/10.1128/iai.00568-08
Abstract
The transformation of the enteropathogenic bacterium Yersinia pseudotuberculosis into the plague bacillus, Yersinia pestis , has been accompanied by extensive genetic loss. This study focused on chromosomal regions conserved in Y. pseudotuberculosis and lost during its transformation into Y. pestis . An extensive PCR screening of 78 strains of the two species identified five regions (R1 to R5) and four open reading frames (ORFs; orf1 to orf4 ) that were conserved in Y. pseudotuberculosis and absent from Y. pestis . Their conservation in Y. pseudotuberculosis suggests a positive selective pressure and a role during the life cycle of this species. Attempts to delete two ORFs ( orf3 and orf4 ) from the chromosome of strain IP32953 were unsuccessful, indicating that they are essential for its viability. The seven remaining loci were individually deleted from the IP32953 chromosome, and the ability of each mutant to grow in vitro and to kill mice upon intragastric infection was evaluated. Four loci ( orf1 , R2, R4, and R5) were not required for optimal growth or virulence of Y. pseudotuberculosis . In contrast, orf2 , encoding a putative pseudouridylate synthase involved in RNA stability, was necessary for the optimal growth of IP32953 at 37°C in a chemically defined medium (M63S). Deletion of R1, a region predicted to encode the methionine salvage pathway, altered the mutant pathogenicity, suggesting that the availability of free methionine is severely restricted in vivo. R3, a region composed mostly of genes of unknown functions, was necessary for both optimal growth of Y. pseudotuberculosis at 37°C in M63S and for virulence. Therefore, despite their loss in Y. pestis , five of the nine Y. pseudotuberculosis -specific chromosomal loci studied play a role in the survival, growth, or virulence of this species.Keywords
This publication has 58 references indexed in Scilit:
- The Complete Genome Sequence of Yersinia pseudotuberculosis IP31758, the Causative Agent of Far East Scarlet-Like FeverPLoS Genetics, 2007
- Complete Genome Sequence of Yersinia pestis Strains Antiqua and Nepal516: Evidence of Gene Reduction in an Emerging PathogenJournal of Bacteriology, 2006
- The Complete Genome Sequence and Comparative Genome Analysis of the High Pathogenicity Yersinia enterocolitica Strain 8081PLoS Genetics, 2006
- Evaluation of O-antigen inactivation on Pla activity and virulence of Yersinia pseudotuberculosis harbouring the pPla plasmidMicrobiology, 2005
- The pseudouridine synthase RluD is required for normal ribosome assembly and function in Escherichia coliRNA, 2005
- Genome sequence of Yersinia pestis, the causative agent of plagueNature, 2001
- The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factorStructure, 1997
- Adhesive properties conferred by the plasminogen activator of Yersinia pestisJournal of General Microbiology, 1992
- A new family of bacterial regulatory proteinsFEMS Microbiology Letters, 1991
- A highly efficient electroporation system for transformation of YersiniaGene, 1990