Transfection with plasmid pSV2gptEJ induces chromosome rearrangements in CHEF cells.
- 1 January 1987
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 84 (1), 184-188
- https://doi.org/10.1073/pnas.84.1.184
Abstract
In previous cytogenetic studies, trisomy for 3q was found to be the most frequent chromosome change associated with induced tumorigenicity by a variety of agents in Chinese hamster cells. Here we describe similar chromosome changes in 11 lines of CHEF/18 cells transfected with the mutant c-Ha-ras containing plasmid pSV2gptEJ. All 11 lines contained the transfected EJ gene and expressed increased levels of p21, the EJ gene product. Ten of the 11 lines were tumorigenic and all but 2 of these were trisomic for all or part of 3q. One line remained diploid and was nontumorigenic despite expressing elevated p21. Two tumorigenic lines from "hit-and-run" transfection with pSV2gpt were shown to express only control levels of p21, but they were trisomic for 3q. These results show that increased p21 expression is neither necessary nor sufficient for inducing tumorigenicity of CHEF cells. We propose that tumorigenicity in the transfected CHEF/18 cells of this study was induced by chromosome rearrangements, especially trisomy for 3q, that occurred at increased frequencies following transfection with pSV2gptEJ.Keywords
This publication has 16 references indexed in Scilit:
- Spontaneous in vitro neoplastic evolution: Recurrent chromosome changes of newly immortalized Chinese hamster cellsCancer Genetics and Cytogenetics, 1986
- Nonrandom loss of chromosome 15 in Syrian hamster tumours induced by v-Ha-ras plus v-myc oncogenesNature, 1985
- THE MOLECULAR GENETICS OF CELLULAR ONCOGENESAnnual Review of Genetics, 1984
- Genetic analysis of tumerigenesis: XVI. Chromosome changes in azacytidine- and insulin-induced tumorigenesisSomatic Cell and Molecular Genetics, 1984
- Loss of the Y chromosome in a cultured human salivary‐gland adenocarcinomaJournal of Oral Pathology & Medicine, 1983
- CELLULAR ONCOGENES AND RETROVIRUSESAnnual Review of Biochemistry, 1983
- Genetic analysis of tumorigenesis: XII. Genetic control of the anchorage requirement in CHEF cellsSomatic Cell and Molecular Genetics, 1982
- Isolation of a transforming sequence from a human bladder carcinoma cell lineCell, 1982
- Genetic analysis of tumorigenesis: VI. Chromosome rearrangements in tumors derived from diploid premalignant Chinese hamster cells in nude miceSomatic Cell and Molecular Genetics, 1980
- Genetic analysis of tumorigenesis: I. Expression of tumor-forming ability in hamster hybrid cell linesSomatic Cell and Molecular Genetics, 1978