Demonstration That Antigen-Binding Cells Are Precursors of Antibody-Producing Cells After Purification with a Fluorescence-Activated Cell Sorter
Open Access
- 1 July 1972
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 69 (7), 1934-1938
- https://doi.org/10.1073/pnas.69.7.1934
Abstract
We have obtained viable and functional populations of antigen-binding cells enriched up to 500-fold from primed spleen-cell suspensions by fluorescent labeling and by a new electronic cell sorter that sorts viable cells according to fluorescence. Concomitantly, populations largely depleted of antigen-binding cells were obtained. While neither population alone is capable of a full adoptive secondary response when injected into irradiated recipients, a reconstituted mixture restores the full response of the unfractionated spleen cells. Admixture of sources of unprimed thymus-derived cells (T-cells) with the purified antigen-binding cells (B-cells) restores much of the full response.Keywords
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