High-titred neutralizing antibodies to human enterovirus 71 preferentially bind to the N-terminal portion of the capsid protein VP1
- 23 February 2007
- journal article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 152 (6), 1069-1073
- https://doi.org/10.1007/s00705-007-0941-1
Abstract
Human enterovirus 71 has emerged as an important pathogen of children in the Asia Pacific region, and it may be important to consider the development of a vaccine against this virus. Human cord serum was used as a source of neutralizing antibodies to determine whether the N- or C-terminal half of the VP1 capsid protein was more likely to harbour neutralizing determinants. Cord sera from 205 individuals were tested for neutralizing antibodies against human enterovirus 71 in an indirect ELISA against recombinant VP1 antigen as well as the N- and C-terminal portions of VP1 antigen. High-titred human neutralizing antibodies were significantly more reactive with the N-terminal half of VP1 than weak or negative sera. The N-terminal half of human enterovirus 71 is likely to have important neutralizing antibody determinants and should be investigated further in vaccine development efforts.Keywords
This publication has 10 references indexed in Scilit:
- Frequent Importation of Enterovirus 71 from Surrounding Countries into the Local Community of Yamagata, Japan, between 1998 and 2003Journal of Clinical Microbiology, 2005
- Molecular Epidemiology of Human Enterovirus 71 Strains and Recent Outbreaks in the Asia-Pacific Region: Comparative Analysis of the VP1 and VP4 GenesEmerging Infectious Diseases, 2003
- An overview of the evolution of enterovirus 71 and its clinical and public health significanceFEMS Microbiology Reviews, 2002
- Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virusVaccine, 2001
- Phylogenetic Analysis of Enterovirus 71 Strains Isolated during Linked Epidemics in Malaysia, Singapore, and Western AustraliaJournal of Virology, 2001
- The N-Terminal Region of the VP1 Protein of Swine Vesicular Disease Virus Contains a Neutralization Site That Arises upon Cell Attachment and Is Involved in Viral EntryJournal of Virology, 2001
- Neutralizing antibody provided protection against enterovirus type 71 lethal challenge in neonatal miceJournal of Biomedical Science, 2000
- Induction of neutralizing antibodies by synthetic peptides representing the C terminus of coxsackievirus A9 capsid protein VP1.Journal of General Virology, 1998
- Identification of the location of antigenic sites of swine vesicular disease virus with neutralization-resistant mutantsJournal of General Virology, 1995
- Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.Proceedings of the National Academy of Sciences of the United States of America, 1983