Single-Cell Gene Expression Profiling
- 2 August 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 297 (5582), 836-840
- https://doi.org/10.1126/science.1072241
Abstract
A key goal of biology is to relate the expression of specific genes to a particular cellular phenotype. However, current assays for gene expression destroy the structural context. By combining advances in computational fluorescence microscopy with multiplex probe design, we devised technology in which the expression of many genes can be visualized simultaneously inside single cells with high spatial and temporal resolution. Analysis of 11 genes in serum-stimulated cultured cells revealed unique patterns of gene expression within individual cells. Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: “cellular genomics.”Keywords
This publication has 14 references indexed in Scilit:
- The Transcriptional Program in the Response of Human Fibroblasts to SerumScience, 1999
- Visualization of Single RNA Transcripts in SituScience, 1998
- Expression monitoring by hybridization to high-density oligonucleotide arraysNature Biotechnology, 1996
- Multicolor Spectral Karyotyping of Human ChromosomesScience, 1996
- Karyotyping human chromosomes by combinatorial multi-fluor FISHNature Genetics, 1996
- Rat model contributes new loci for NIDDM susceptibility in manNature Genetics, 1996
- Serial Analysis of Gene ExpressionScience, 1995
- Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA MicroarrayScience, 1995
- Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain ReactionScience, 1992
- PRIMARY RESPONSE GENES INDUCED BY GROWTH FACTORS AND TUMOR PROMOTERSAnnual Review of Biochemistry, 1991