Immunohistochemical detection of proliferating cell nuclear antigen in hepatocellular carcinoma: Relationship to histological grade
- 1 October 1993
- journal article
- Published by Wiley in Journal of Gastroenterology and Hepatology
- Vol. 8 (5), 420-425
- https://doi.org/10.1111/j.1440-1746.1993.tb01541.x
Abstract
Proliferating cell nuclear antigen (PCNA), also known as cyclin, is an auxiliary protein of DNA polymerase‐δ and is found only in the nuclei of proliferating cells in the late G1 and S phases. The proliferation of hepatocellular carcinoma (HCC) by immunohistochemical staining for PCNA using paraffin sections of 20 surgically resected HCC specimens was analysed. The mean percentage of PCNA‐positive nuclei in the HCC tissue was 10.3% in grade I of Edmondson and Steiner's classification, 25.5% in grade II, 28.4% in grade III and 41.5% in grade IV. In early HCC, we observed only a few PCNA‐positive tumour cells. However, PCNA‐positive nuclei were numerous in the tumour thrombi found in portal vein branches, in regions of extracapsular tumour growth, and in the inner nodules of tumours with a nodule‐in‐nodule formation. Proliferating cell nuclear antigen positivity was correlated with an increase of the nucleocytoplasmic ratio of tumour cells as determined by image analysis. Our findings showed that PCNA positivity was correlated with the histological grade and invasiveness of HCC, suggesting that this antigen may be used as an indicator to predict tumour invasion in patients with HCC.Keywords
This publication has 16 references indexed in Scilit:
- PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines.Journal of Histochemistry & Cytochemistry, 1991
- Immunohistological detection of proliferating cells using anti-PCNA monoclonal antibody in paraffin-embedded liver needle-biopsied specimens.Kanzo, 1991
- Heterogeneity of Proliferative Activity in Nodule‐in‐Nodule Lesions of Small Hepatocellular CarcinomaJapanese Journal of Cancer Research, 1990
- A histopathologic study of early hepatocellular carcinoma (HCC): Portal tract invasion and progression to advanced HCCKanzo, 1990
- A morphometrical study of well-differentiated hepatocellular carcinoma. Diagnostic usefulness of the nuclear roundness factor.Kanzo, 1990
- Cyclin/PCNA is the auxiliary protein of DNA polymerase-δNature, 1987
- Prognostic significance of DNA-analysis by flow cytometry in non-Hodgkin's lymphomaHematological Oncology, 1985
- Antibodies to Human c- myc Oncogene Product: Evidence of an Evolutionarily Conserved Protein Induced During Cell ProliferationScience, 1984
- Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferationInternational Journal of Cancer, 1983
- A nuclear antigen associated with cell proliferation and blast transformation.The Journal of Experimental Medicine, 1981