Logarithmic intensity and speckle-based motion contrast methods for human retinal vasculature visualization using swept source optical coherence tomography
Open Access
- 10 February 2012
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 3 (3), 503-521
- https://doi.org/10.1364/boe.3.000503
Abstract
We formulate a theory to show that the statistics of OCT signal amplitude and intensity are highly dependent on the sample reflectivity strength, motion, and noise power. Our theoretical and experimental results depict the lack of speckle amplitude and intensity contrasts to differentiate regions of motion from static areas. Two logarithmic intensity-based contrasts, logarithmic intensity variance (LOGIV) and differential logarithmic intensity variance (DLOGIV), are proposed for serving as surrogate markers for motion with enhanced sensitivity. Our findings demonstrate a good agreement between the theoretical and experimental results for logarithmic intensity-based contrasts. Logarithmic intensity-based motion and speckle-based contrast methods are validated and compared for in vivo human retinal vasculature visualization using high-speed swept-source optical coherence tomography (SS-OCT) at 1060 nm. The vasculature was identified as regions of motion by creating LOGIV and DLOGIV tomograms: multiple B-scans were collected of individual slices through the retina and the variance of logarithmic intensities and differences of logarithmic intensities were calculated. Both methods captured the small vessels and the meshwork of capillaries associated with the inner retina in en face images over 4 mm2 in a normal subject.Keywords
This publication has 26 references indexed in Scilit:
- Intensity-based modified Doppler variance algorithm: application to phase instable and phase stable optical coherence tomography systemsOptics Express, 2011
- Real-time bulk-motion-correction free Doppler variance optical coherence tomography for choroidal capillary vasculature imagingOptics Express, 2011
- Comprehensive in vivo micro-vascular imaging of the human eye by dual-beam-scan Doppler optical coherence angiographyOptics Express, 2011
- Multifunctional imaging of human retina and choroid with 1050-nm spectral domain optical coherence tomography at 92-kHz line scan rateJournal of Biomedical Optics, 2011
- Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imagingNature Medicine, 2009
- Three-dimensional quantitative imaging of retinal and choroidal blood flow velocity using joint Spectral and Time domain Optical Coherence TomographyOptics Express, 2009
- Optical coherence angiographyOptics Express, 2006
- In vivo optical frequency domain imaging of human retina and choroidOptics Express, 2006
- Speckle reduction in optical coherence tomography by frequency compoundingJournal of Biomedical Optics, 2003
- Retinal blood flow in diabetic retinopathy.BMJ, 1992