The Association between Plasma 25-Hydroxyvitamin D and Subgroups in Age-Related Macular Degeneration: A Cross-Sectional Study
Open Access
- 29 July 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (7), e70948
- https://doi.org/10.1371/journal.pone.0070948
Abstract
To evaluate potential differences in plasma 25-hydroxyvitamin in subtypes of age-related macular degeneration (AMD), and in patients in Clinical Age-Related Maculopathy Staging (CARMS) group 5 with or without subretinal fibrosis. This single-center cross-sectional study included 178 participants during a period of 20 months. Ninety-five patients belonged to CARMS 5; twelve belonged to CARMS 4; twenty-two belonged to CARMS 2 or 3; and 49 individuals did not have AMD (CARMS 1). Following a structured interview, a detailed bilateral retinal examination was performed and participants were allocated to their respective subgroups in accordance with the Clinical Age-Related Maculopathy Staging system. Plasma 25-hydroxyvitamin D2 and D3 were analyzed using liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from leukocytes and genotyped for single nucleotide polymorphisms (SNPs) in the vitamin D metabolism. Differences in plasma 25-hydroxyvitamin D were determined in the subgroups as well as between patients in CARMS 5 with or without subretinal fibrosis. Plasma 25-hydroxyvitamin D was comparable in patients across CARMS groups 1 to 5 (p = 0.83). In CARMS 5, the presence of subretinal fibrosis was associated with significantly lower concentrations of 25-hydroxyvitamin D as compared to the absence of subretinal fibrosis (47.2 versus 75.6 nmol/L, p<0.001). Patients in CARMS 5 with subretinal fibrosis were more likely to have insufficient levels of 25-hydroxyvitamin D compared to patients without subretinal fibrosis (p = 0.006). No association was found between the SNPs rs10877012, rs2228570, rs4588, or rs7041 and AMD subgroups or plasma 25-hydroxyvitamin levels. This study suggests that the presence of subretinal fibrosis in patients belonging to CARMS 5 may be associated with a poor vitamin D status. Our observations warrant further investigation into the role of vitamin D in the development of subretinal fibrosis.Keywords
This publication has 53 references indexed in Scilit:
- Intraindividual Variation in Plasma 25-Hydroxyvitamin D Measures 5 Years Apart among Postmenopausal WomenCancer Epidemiology, Biomarkers & Prevention, 2012
- Reconsidering the connection between vitamin D levels and age-related macular degenerationEye, 2011
- Vitamin D Status and Early Age-Related Macular Degeneration in Postmenopausal WomenJAMA Ophthalmology, 2011
- 1,25-Dihydroxyvitamin D3 suppresses inflammation-induced expression of plasminogen activator inhibitor-1 by blocking nuclear factor-κB activationArchives of Biochemistry and Biophysics, 2011
- Age-related Macular Degeneration: Genetic and Environmental Factors of DiseaseMolecular Interventions, 2010
- Macrophages: Master Regulators of Inflammation and FibrosisSeminars in Liver Disease, 2010
- Vitamin D inhibition of pro-fibrotic effects of transforming growth factor β1 in lung fibroblasts and epithelial cellsThe Journal of Steroid Biochemistry and Molecular Biology, 2010
- Quantitative Optical Coherence Tomography Findings in Various Subtypes of Neovascular Age-Related Macular DegenerationInvestigative Ophthalmology & Visual Science, 2008
- Cigarette smoking and subsequent risk of lung cancer in men and women: analysis of a prospective cohort studyThe Lancet Oncology, 2008
- Transforming growth factor-β in T-cell biologyNature Reviews Immunology, 2002