Actin Filament Dynamics in Living Glial Cells Imaged by Atomic Force Microscopy
- 25 September 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 257 (5078), 1944-1946
- https://doi.org/10.1126/science.1411511
Abstract
Observation of filamentous actin (F-actin) in living cells is currently limited to the resolution of the light microscope. Higher resolution procedures require sample fixation and preclude dynamic studies. The atomic force microscope (AFM) can image and manipulate samples at very high, sometimes atomic resolution by scanning a fine tip over the surface of interest and detecting physical interactions between the tip and sample. This study demonstrates that F-actin can be readily resolved in living cells with the AFM and that the dynamic properties of F-actin are easily observed.Keywords
This publication has 19 references indexed in Scilit:
- Atomic force microscopy for high-resolution imaging in cell biologyTrends in Cell Biology, 1992
- Circular DNA molecules imaged in air by scanning force microscopyBiochemistry, 1992
- Does the Scanning Force Microsope Resolve Individual Lipid Molecules?Springer Proceedings in Physics, 1992
- Three-Dimensional Readout of Flash X-Ray Images of Living Sperm in Water by Atomic-Force MicroscopyScience, 1991
- Molecular-resolution images of Langmuir-Blodgett films and DNA by atomic force microscopyLangmuir, 1991
- Imaging the membrane protein bacteriorhodopsin with the atomic force microscopeBiophysical Journal, 1990
- Imaging cells with the atomic force microscopeJournal of Structural Biology, 1990
- Imaging Crystals, Polymers, and Processes in Water with the Atomic Force MicroscopeScience, 1989
- Neuronal Cytomechanics: The Actin-Based Motility of Growth ConesScience, 1988
- Atomic Force MicroscopePhysical Review Letters, 1986