Rapid and reliable high‐throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms
- 11 June 2010
- journal article
- Published by Wiley in Molecular Ecology Resources
- Vol. 10 (4), 628-633
- https://doi.org/10.1111/j.1755-0998.2009.02825.x
Abstract
We present two methods for DNA extraction from fresh and dried mushrooms that are adaptable to high-throughput sequencing initiatives, such as DNA barcoding. Our results show that these protocols yield ∼85% sequencing success from recently collected materials. Tests with both recent (100 years) specimens reveal that older collections have low success rates and may be an inefficient resource for populating a barcode database. However, our method of extracting DNA from herbarium samples using small amount of tissue is reliable and could be used for important historical specimens. The application of these protocols greatly reduces time, and therefore cost, of generating DNA sequences from mushrooms and other fungi vs. traditional extraction methods. The efficiency of these methods illustrates that standardization and streamlining of sample processing should be shifted from the laboratory to the field.Keywords
This publication has 16 references indexed in Scilit:
- Express barcodes: racing from specimen to identificationMolecular Ecology Resources, 2009
- Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversityMolecular Ecology Resources, 2009
- Progress towards DNA barcoding of fungiMolecular Ecology Resources, 2009
- The front‐end logistics of DNA barcoding: challenges and prospectsMolecular Ecology Resources, 2009
- DNA Barcoding—a Windfall for Tropical Biology?Biotropica, 2008
- A high‐throughput protocol for extracting high‐purity genomic DNA from plants and animalsMolecular Ecology Resources, 2008
- An inexpensive, automation‐friendly protocol for recovering high‐quality DNAMolecular Ecology Notes, 2006
- Biological identifications through DNA barcodesProceedings. Biological sciences, 2003
- The Importance of Being Earnest: What, if Anything, Constitutes a “Specimen Examined?”Molecular Phylogenetics and Evolution, 2000
- Amplification and Sequencing of DNA from Fungal Herbarium SpecimensMycologia, 1990