Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types
Open Access
- 1 June 2005
- journal article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 58 (6), 621-625
- https://doi.org/10.1136/jcp.2004.021774
Abstract
Background: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p < 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription.Keywords
This publication has 44 references indexed in Scilit:
- Postgenomic global analysis of translational control induced by oncogenic signalingOncogene, 2004
- The Polypeptide Chain-releasing Factor GSPT1/eRF3 Is Proteolytically Processed into an IAP-binding ProteinJournal of Biological Chemistry, 2003
- A Novel Role of the Mammalian GSPT/eRF3 Associating with Poly(A)-binding Protein in Cap/Poly(A)-dependent TranslationJournal of Biological Chemistry, 2002
- Clinical implications of chromosomal abnormalities in gastric adenocarcinomasGenes, Chromosomes and Cancer, 2002
- Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulationCell Motility, 2002
- Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancerNature Genetics, 2002
- Assignment1 of the human peptide chain release factor 3 (GSPT2) to Xp11.23→p11.21 and of the distal marker DXS1039 by radiation hybrid mappingCytogenetic and Genome Research, 1999
- The Eukaryotic Polypeptide Chain Releasing Factor (eRF3/GSPT) Carrying the Translation Termination Signal to the 3′-Poly(A) Tail of mRNAJournal of Biological Chemistry, 1999
- Depletion of aDrosophila homolog of yeast Sup35p disrupts spindle assembly, chromosome segregation, and cytokinesis during male meiosisCell Motility, 1998
- Mapping of the humanGSPT1 gene, a human homolog of the yeastGST1 gene, to chromosomal band 16p13.1Somatic Cell and Molecular Genetics, 1992