Doğal Bir Hyaluronik Asit Kaynağı Olarak Sinovyal Sıvının Limberg Flep ve Eliptik Rotasyon Flep İyileşmesi Üzerine Etkisi: Farelerde Tam Katmanlı Eksizyonel Dermal Yaralarda Karşılaştırmalı Bir Çalışma

Abstract

The purpose of this study was to investigate the efficacy of synovial fluid (SF) as a source of hyaluronic acid (HA) on Limberg and Elliptical rotation flap healing. In this study conducted on 24 male Mus musculus mice, the animals were divided into 2 main groups. Group 1 (n = 12): Limbergflap group; Group 2 (n = 12) Elliptical rotation flap group. Six of the animals in Group 1 and Group 2 were assigned as the Flap (Control) Group, and 6 as the Flap + SF Group. In SF subgroups of animals in each group, 1 mL SF was injected into the operation area in addition to the flap surgical procedure. The groups were compared in terms of epithelialization, angiogenesis, neutrophil leukocyte infiltration and fibroblast activity, and the effect of SF on the inflammation and wound healing was evaluated. It was observed that epithelization was completed in all animals in the groups which were administered compared to the other groups, and there was a statistically significant difference (P<0.05). In addition, no necrosis or abscess was found in the groups which were administered SF. There was no statistical difference in the paired comparisons of neutrophil-leukocyte infiltration and fibroblast activity parameters. Although the Limberg flap technique is more commonly used today, it can be said that elliptical rotation flap is a method that can be used in routine practice due to a lower complication rate. However, SF increases epithelization and plays a regulatory role on angiogenesis. Therefore, it is concluded that the use of SF as a source of HA in combination with elliptical rotation flap in wounds with material tissue loss will contribute to clinical practice.