Pre‐Clinical Mouse Models of Primary and Metastatic Pleural Cancers of the Lung and Breast and the Use of Bioluminescent Imaging to Monitor Pleural Tumor Burden
Open Access
- 1 September 2011
- journal article
- unit
- Published by Wiley in Current Protocols in Pharmacology
- Vol. 54 (1), 14.21.1-14.21.18
- https://doi.org/10.1002/0471141755.ph1421s54
Abstract
Malignant pleural disease (MPD) results in an estimated 150,000 cases of malignant pleural effusions (MPE) annually. The most common malignancies associated with MPD are primary malignant pleural mesothelioma (MPM) and metastatic lung cancer, breast cancer, and lymphoma. MPM is a rare, regionally aggressive malignancy whose incidence is increasing secondarily to the latency of disease progression. MPD is characteristic of advanced‐stage pleural disease and portends a grave clinical prognosis with a median survival between 3 and 12 months. Preclinical investigations conducted in flank and intraperitoneal tumor models do not fully recapitulate the pleural tumor microenvironment, and the results are not directly translatable to the clinical setting. The protocol described herein provides a mouse model of MPM and MPD from nonhematogenous tumors, resulting in reproducible tumor location, tumor progression, animal survival, and histopathology. Pleural tumor growth in this model resembles the regionally aggressive clinical course and tumor microenvironment of human pleural cancers and provides an optimal animal model to investigate MPD biology and therapies. Curr. Protoc. Pharmacol . 54:14.21.1‐14.21.18. © 2011 by John Wiley & Sons, Inc.Keywords
This publication has 18 references indexed in Scilit:
- Animal models of malignant pleural effusionCurrent Opinion in Pulmonary Medicine, 2009
- Techniques in Aseptic Rodent SurgeryCurrent Protocols in Immunology, 2008
- Intraoperative localization of lymph node metastases with a replication-competent herpes simplex virusThe Journal of Thoracic and Cardiovascular Surgery, 2006
- EuthanasiaCurrent Protocols in Immunology, 2006
- Imaging and therapy of malignant pleural mesothelioma using replication‐competent herpes simplex virusesThe Journal of Gene Medicine, 2006
- Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancerCancer Gene Therapy, 2005
- Visceral pleural invasion is an invasive and aggressive indicator of non-small cell lung cancerThe Journal of Thoracic and Cardiovascular Surgery, 2005
- Visceral pleural involvement in nonsmall cell lung cancer: prognostic significanceThe Annals of Thoracic Surgery, 2004
- BTS guidelines for the management of malignant pleural effusionsThorax, 2003
- Malignant Pleural EffusionsSeminars in Respiratory and Critical Care Medicine, 2001