Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes
- 1 May 2007
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 213 (1), 201-211
- https://doi.org/10.1002/jcp.21113
Abstract
Cumulus cell layers of expanded cumulus oocyte complexes (COCs) are interlinked with networks of hyaluronic acid, chondroitin sulfate B proteoglycans and link proteins, and they can be dispersed by sperm surface hyaluronidases. In this report, we showed that arylsulfatase A (AS-A), existing on the sperm head surface, also had this dispersion action. Purified AS-A free of protease, hyaluronidase and chondroitinase activities could disperse the cumulus matrix of expanded COCs. However, this COC dispersion action was not associated with AS-A desulfation activity, assayed by using p-nitrocatecholsulfate (artificial substrate). COCs incubated for 1 h with sperm pretreated with anti-AS-A IgG in the presence of apigenin (a hyaluronidase inhibitor) did not exhibit matrix dispersion, whereas several cumulus layers were already dispersed in COCs incubated with sperm pretreated with preimmune IgG. Furthermore, sperm from AS-A null mice showed a significant delay in COC dispersion, compared with wild-type sperm. Within 1 h of sperm-COC co-incubation, the size of COCs incubated with AS-A null sperm was 65% of the original dimension, whereas that of COCs inseminated with wild-type sperm was only 17%. A further delay in COC dispersion by AS-A(−/−) mouse sperm was observed when apigenin was present in the co-incubation. We also showed for the first time that AS-A had a specific affinity for chondroitin sulfate B, a component of cumulus matrix proteoglycan networks; this might provide a mechanism of cumulus matrix destabilization induced by sperm surface AS-A. J. Cell. Physiol. 213: 201–211, 2007.Keywords
This publication has 53 references indexed in Scilit:
- Epididymal SPAM1 Is a Marker for Sperm Maturation in the Mouse1Biology of Reproduction, 2006
- Expression and Purification of Functionally Active Hyaluronan-binding Domains from Human Cartilage Link Protein, Aggrecan and VersicanPublished by Elsevier BV ,2005
- Arylsulfatase A from human placenta possesses only high mannose-type glycansThe International Journal of Biochemistry & Cell Biology, 1997
- Effect of Oocytectomy on Glycosaminoglycan Composition during Cumulus Expansion of Porcine Cumulus-Oocyte Complexes Cultured in Vitro1Biology of Reproduction, 1996
- Sperm Surface Protein PH-20 is Bifunctional: One Activity is a Hyaluronidase and a Second, Distinct Activity is Required in Secondary Sperm-Zona BindingBiology of Reproduction, 1996
- A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg.The Journal of cell biology, 1994
- A substrate-gel assay for hyaluronidase activityMatrix, 1992
- Proteolytic processing of human lysosomal arylsulfatase ABiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Purification of Boar Acrosomal Arylsulfatase A and Possible Role in the Penetration of Cumulus CellsBiology of Reproduction, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970