Substitution of equally carcinogenic UV-A for UV-B irradiations lowers epidermal thyrnine dimer levels during skin cancer induction in hairless mice

Abstract

Cyclobutane pyrimidine diiners (CPD) are the predominant DNA lesions Induced by UV-B radiation, among these lesions thymine dimers are most frequent. Although UV-A radiation may also induce CPD, it has been found that equally cytotoxic or equally mutagemc UV-A and UY-B doses do not Induce equal amounts of CPD, Indicating that other DNA adducts contribute to the UV-A effects. Thus far it has not been established whether this finding can be extrapolated and also holds true for the more complex biological endpoint of skin cancer. Therefore, we compared thymine dimer levels during skin cancer Induction by combined UV-A and UV-B daily exposures with the levels from equally carcinogenic daily UV-B exposures. From control experiments It was known that both groups would react similarly regarding the occurrences of carcinomas, with a median latency time of 170 ± 10 days. After 50, 106 and 151 days of irradiation eight hairless mice (SKH:HIR1) from both groups were euthanized and thymine dimers In epidermal cell suspensions were quantified by flow cytometry. Staining on DNA content enabled us to quantify thymine dimers in G0/G1-phase, in S-phase and in G2M-phase subpopulatlons. Both In total epidermal cell populations and in subpopulatlons of replicating epidermal cells thymine dimer levels were significantly lower In the UV-A/B combination group than in the UV-B group (0.010 <PP<0.005 respectively). This indicates that the carcinogenicity of UV-A relative to that of UV-B Is not properly measured by thymine dlmers and that other DNA lesions than CPD, for example, from reactive oxygen species, are likely to contribute to UV-A carcinogenicity.