The doctor of medicine and philosophy Stefano Lorenzo Bisio (1724–1800?) worked in the Grand Duchy of Lithuania between 1762/3 and 1787. During this quarter of a century, he earned renown as a private physician to magnates, an innovator in the science of medicine, and as one of the founders of academic medicine in the Grand Duchy of Lithuania. Regardless of this, only one of the last stages of Bisio’s career has received much attention in historiography so far, his becoming the first head of the College of Medicine, established at Vilnius University in 1781. In this article, I seek to give as comprehensive as possible a presentation of Bisio’s entire career in the Grand Duchy of Lithuania, discussing thoroughly his academic activities and achievements. Through these achievements, I hope to show that he was one of the first to apply West European anatomical, pathological and clinical medical knowledge from the Age of Enlightenment, which itself was undergoing qualitative breakthroughs, in the GDL. In the article, I also correct and present new biographical facts about S.L. Bisio, his birth and death dates, work and education placesfamily, and work relations.