Direct determination of four fluoroquinolones, enoxacin, norfloxacin, ofloxacin, and ciprofloxacin, in pharmaceuticals and blood serum by HPLC
- 13 February 2003
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 375 (5), 623-629
- https://doi.org/10.1007/s00216-003-1749-9
Abstract
A rapid, accurate and sensitive method has been developed for the quantitative determination of four fluoroquinolone antimicrobial agents, enoxacin, norfloxacin, ofloxacin and ciprofloxacin, with high in-vitro activity against a wide range of Gram-negative and Gram-positive organisms. A Kromasil 100 C8 250 mm×4 mm, 5 μm analytical column was used with an eluting system consisting of a mixture of CH3CN–CH3OH–citric acid 0.4 mol L−1 (7:15:78 %, v/v). Detection was performed with a variable wavelength UV–visible detector at 275 nm resulting in limits of detection: 0.02 ng per 20 μL injection for enoxacin and 0.01 ng for ofloxacin, norfloxacin and ciprofloxacin. Hydrochlorothiazide (HCT) was used as internal standard at a concentration of 2 ng μL−1. A rectilinear relationship was observed up to 2 ng μL−1 for enoxacin, 12 ng μL−1 for ofloxacin, 3 ng μL−1 for norfloxacin, and 5 ng μL−1 for ciprofloxacin. Separation was achieved within 10 min. The statistical evaluation of the method was examined by performing intra-day (n=8) and inter-day precision assays (n=8) and was found to be satisfactory with high accuracy and precision. The method was applied to the direct determination of the four fluoroquinolones in human blood serum. Sample pretreatment involved only protein precipitation with acetonitrile. Recovery of analytes in spiked samples was 97±6% over the range 0.1–0.5 ng μL−1.This publication has 19 references indexed in Scilit:
- Clinical Issues in the Prophylaxis, Diagnosis, and Treatment of AnthraxEmerging Infectious Diseases, 2002
- Determination of quinolones in animal tissues and eggs by high-performance liquid chromatography with photodiode-array detectionJournal of Chromatography A, 2000
- Simultaneous determination of enrofloxacin and ciprofloxacin in animal biological fluids by high-performance liquid chromatography: Application in pharmacokinetic studies in pig and rabbitJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- A Rapid and Simple High Pressure Liquid Chromatographic Method for Pharmacokinetic Study of Ciprofloxacin in Human SerumAnalytical Letters, 1998
- High-performance liquid chromatographic determination of ciprofloxacin in plasmaJournal of Clinical Pharmacy & Therapeutics, 1992
- A simple high-performance liquid chromatographic assay for ciprofloxacin in human serumJournal of Clinical Pharmacy & Therapeutics, 1992
- Determination of ciprofloxacin in human serum by liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1991
- Simultaneous high-performance liquid chromatographic determination of ciprofloxacin, fenbufen and felbinac in rat plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1990
- High-performance liquid chromatographic determination of several quinolone antibacterials in medicated fish feedJournal of Chromatography A, 1990
- A simple isocratic high-pressure liquid chromatographic assay of quinolones in serumJournal of Antimicrobial Chemotherapy, 1989