Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-loci variable-number tandem repeat typing method onMycobacterium tuberculosisstrains predominated by the Beijing family
Open Access
- 1 May 2007
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 270 (1), 67-74
- https://doi.org/10.1111/j.1574-6968.2007.00658.x
Abstract
The newly proposed 15- and 24-loci mycobacterial interspersed repetitive unit (MIRU)–variable-number tandem repeat (VNTR) typing method was evaluated for its ability to differentiate 181 Mycobacterium tuberculosis Beijing family strains. Compared with the original 12-loci MIRU–VNTR typing method, the 15-loci system dramatically improved the discriminatory power for Beijing strains; however, large clusters that could be further differentiated by IS6110 restriction fragment length polymorphism (RFLP) were still obtained. The clonal stability and allelic diversity of a total of 31 VNTR loci were evaluated. VNTRs 3232, 3820, and 4120 were identified as the effective hypervariable VNTR set for the second-line typing of clustered strains following the 15-loci based scheme. Consequently, the discriminatory power of the new scheme (18 loci) equaled that of IS6110 RFLP.Keywords
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