Cell-Mediated Immune Responses to Human Papillomavirus 16 E6 and E7 Antigens as Measured by Interferon Gamma Enzyme-Linked Immunospot in Women With Cleared or Persistent Human Papillomavirus Infection
- 1 May 2009
- journal article
- Published by BMJ in International Journal of Gynecologic Cancer
- Vol. 19 (4), 508-512
- https://doi.org/10.1111/igc.0b013e3181a388c4
Abstract
Cell-mediated immune responses have been thought to be important in the control of human papillomavirus (HPV) infections. We examined cell-mediated immune responses to HPV-16 E6 and E7 in the peripheral blood using interferon gamma (IFN-γ) enzyme-linked immunospot assay (Cellular Technology Ltd, Cleveland, Ohio) in women with HPV-16 infection who showed clearance and compared these women to women with HPV-16 persistence. Women participating in a longitudinal study of cervical HPV were recruited once cervical HPV-16 infection was detected by polymerase chain reaction. Four groups of women were examined: (1) persistent, (2) intermittent, (3) transient, and (4) cleared. Ninety-six samples from 55 women were compared. Comparing IFN-γ enzyme-linked immunospot to the HPV-16 clearance of 10 women with recent persistence, none had response to either E6 or E7; of 24 women with recent clearance, 14 had E6 and 8 had E7 response. Women with intermittent persistence behaved similarly to the clearance group than recent persistors: 50% were positive to E6 and 20% to E7. In summary, anti-E6 responses seem critical in the immediate control of HPV, and in some women, an immune tolerance eventually develops if HPV is not eliminated soon after infection.Keywords
This publication has 23 references indexed in Scilit:
- Vaccination trial with HPV16 L1E7 chimeric virus‐like particles in women suffering from high grade cervical intraepithelial neoplasia (CIN 2/3)International Journal of Cancer, 2007
- Anti-cancer activity of plant-produced HPV16 E7 vaccineVaccine, 2007
- Human papillomavirus type 16 E2- and L1-specific serological and T-cell responses in women with vulval intraepithelial neoplasiaJournal of General Virology, 2003
- Epidemiologic Classification of Human Papillomavirus Types Associated with Cervical CancerThe New England Journal of Medicine, 2003
- Serum Immunoglobulin G Response to Human Papillomavirus Type 16 Virus‐Like Particles in Human Immunodeficiency Virus (HIV)–Positive and Risk‐Matched HIV‐Negative WomenThe Journal of Infectious Diseases, 2003
- A population‐based prospective study of Chlamydia trachomatis infection and cervical carcinomaInternational Journal of Cancer, 2002
- Cell-Mediated Immune Response to Human Papillomavirus InfectionClinical and Diagnostic Laboratory Immunology, 2001
- Persistence of Human Papillomavirus Type 16 Infection Is Associated with Lack of Cytotoxic T Lymphocyte Response to the E6 AntigensThe Journal of Infectious Diseases, 2000
- Detection Of High-Risk Human Papillomavirus In The Cervix And Semen Of Sex PartnersThe Journal of Infectious Diseases, 1994
- Detection of Papillomavirus DNA in Human SemenScience, 1986