Epigenetic Control of Gonadal Aromatase (cyp19a1) in Temperature-Dependent Sex Determination of Red-Eared Slider Turtles
Open Access
- 7 June 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (6), e63599
- https://doi.org/10.1371/journal.pone.0063599
Abstract
In the red-eared slider turtle (Trachemys scripta), a species with temperature-dependent sex determination (TSD), the expression of the aromatase gene during gonad development is strictly limited to the female-producing temperature. The underlying mechanism remains unknown. In this study, we identified the upstream 5′-flanking region of the aromatase gene, gonad-specific promoter, and the temperature-dependent DNA methylation signatures during gonad development in the red-eared slider turtle. The 5′-flanking region of the slider aromatase exhibited sequence similarities to the aromatase genes of the American alligator, chicken, quail, and zebra finch. A putative TATA box was located 31 bp upstream of the gonad-specific transcription start site. DNA methylation at the CpG sites between the putative binding sites of the fork head domain factor (FOX) and vertebrate steroidogenic factor 1 (SF1) and adjacent TATA box in the promoter region were significantly lower in embryonic gonads at the female-producing temperature compared the male-producing temperature. A shift from male- to female-, but not from female- to male-, producing temperature changed the level of DNA methylation in gonads. Taken together these results indicate that the temperature, particularly female-producing temperature, allows demethylation at the specific CpG sites of the promoter region which leads the temperature-specific expression of aromatase during gonad development.Keywords
This publication has 68 references indexed in Scilit:
- Sequencing three crocodilian genomes to illuminate the evolution of archosaurs and amniotesGenome Biology, 2012
- DNA Methylation of the Gonadal Aromatase (cyp19a) Promoter Is Involved in Temperature-Dependent Sex Ratio Shifts in the European Sea BassPLoS Genetics, 2011
- DMRT1 prevents female reprogramming in the postnatal mammalian testisNature, 2011
- Aromatase Is a Direct Target of FOXL2: C134W in Granulosa Cell Tumors via a Single Highly Conserved Binding Site in the Ovarian Specific PromoterPLOS ONE, 2010
- Heat shock factors: integrators of cell stress, development and lifespanNature Reviews Molecular Cell Biology, 2010
- Distinct Transcriptional Mechanisms Direct Expression of the Rat Dmrt1 Promoter in Sertoli Cells and Germ Cells of Transgenic Mice1Biology of Reproduction, 2009
- Developmental programming of CpG island methylation profiles in the human genomeNature Structural & Molecular Biology, 2009
- Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liverGenome Research, 2009
- Atrazine-Induced Aromatase Expression Is SF-1 Dependent: Implications for Endocrine Disruption in Wildlife and Reproductive Cancers in HumansEnvironmental Health Perspectives, 2007
- DNA methylation patterns and epigenetic memoryGenes & Development, 2002