Heterogeneity of bronchial endothelial cell permeability
- 1 September 2002
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Lung Cellular and Molecular Physiology
- Vol. 283 (3), L520-L527
- https://doi.org/10.1152/ajplung.00451.2001
Abstract
In vivo models of airway inflammation suggest that most protein transudation occurs from bronchial microcirculation. However, due to technical limitations in the isolation and culture of bronchial endothelial cells, most studies of lung vascular permeability have focused on pulmonary endothelium. Thus conditions for culture of sheep bronchial artery endothelial cells (BAEC) and bronchial microvascular endothelial cells (BMVEC) were established. The bronchial artery and the mainstem bronchi, stripped of epithelium, were dissected, and endothelial cells were isolated by enzymatic treatment. BAEC and BMVEC demonstrated positive staining for factor VIII-related antigen, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate-labeled low-density lipoprotein, and PECAM-1. Radioligand binding studies confirmed equivalent numbers of bradykinin B2 receptors on BAEC and BMVEC. Permeability of BAEC and BMVEC was determined after treatment with bradykinin and thrombin by comparing the translocation of FITC-dextran (mol wt 9,500) across confluent monolayers ( n = 10–12). Bradykinin caused a maximal increase in permeability in BAEC (165% increase) and BMVEC (144% increase) by 15 min compared with vehicle controls. Thrombin treatment altered BMVEC permeability only, reaching a maximal response at 60 min (109% increase). These results demonstrate bronchial endothelial cell heterogeneity and establish methods to determine intracellular mechanisms contributing to airway disease in relevant cell systems.Keywords
This publication has 31 references indexed in Scilit:
- ENDOTHELIAL CELLS IN CULTURE: A MODEL FOR STUDYING VASCULAR FUNCTIONSPharmacological Research, 2000
- Effect of Vascular Endothelial Growth Factor on Cultured Endothelial Cell Monolayer Transport PropertiesMicrovascular Research, 2000
- Bradykinin and α-Thrombin Increase Human Umbilical Vein Endothelial Macromolecular Permeability by Different MechanismsInflammation, 2000
- Endothelial Cells from Diverse Tissues Exhibit Differences in Growth and MorphologyMicrovascular Research, 1998
- Bradykinin- and thrombin-induced increases in endothelial permeability occur independently of phospholipase C but require protein kinase C activationJournal of Cellular Physiology, 1997
- Interaction between airway edema and lung inflation on responsiveness of individual airways in vivo.Journal of Applied Physiology, 1997
- Dissociation of bradykinin-induced plasma exudation and reactivity in the peripheral airways.American Journal of Respiratory and Critical Care Medicine, 1996
- Airway edema potentiates airway reactivityJournal of Applied Physiology, 1995
- Culture and characterization of pulmonary microvascular endothelial cellsIn Vitro Cellular & Developmental Biology – Animal, 1992
- Organ-derived microvessel endothelial cells exhibit differential responsiveness to thrombin and other growth factorsMicrovascular Research, 1992