Electrochemical immunosensor with NiAl-layered double hydroxide/graphene nanocomposites and hollow gold nanospheres double-assisted signal amplification
- 24 March 2015
- journal article
- Published by Springer Science and Business Media LLC in Bioprocess and Biosystems Engineering
- Vol. 38 (8), 1455-1468
- https://doi.org/10.1007/s00449-015-1388-5
Abstract
A sensitive electrochemical immunosensor based on NiAl-layered double hydroxide/graphene nanocomposites (NiAl-LDH/G) and hollow gold nanospheres (HGNs) was proposed for chlorpyrifos detection. The NiAl-LDH/G was prepared using a conventional coprecipitation process and reduction of the supporting graphene oxide. Subsequently, the nanocomposites were dispersed with chitosan (CS). The NiAl-LDH/G possessed good electrochemical behavior and high binding affinity to the electrode. The high surface areas of HGNs and the vast aminos and hydroxyls of CS provided a platform for the covalently crosslinking of antibody. Under optimal conditions, the immunosensor exhibited a wide linear range from 5 to 150 μg/mL and from 150 to 2 μg/mL, with a detection limit of 0.052 ng/mL. The detection results showed good agreement with standard gas chromatography method. The constructed immunosensor exhibited good reproducibility, high specificity, acceptable stability and regeneration performance, which provided a new promising tool for chlorpyrifos detection in real samples.Keywords
This publication has 34 references indexed in Scilit:
- Increased Electrocatalyzed Performance through Dendrimer-Encapsulated Gold Nanoparticles and Carbon Nanotube-Assisted Multiple Bienzymatic Labels: Highly Sensitive Electrochemical Immunosensor for Protein DetectionAnalytical Chemistry, 2013
- Acetylcholinesterase biosensor based on 3-carboxyphenylboronic acid/reduced graphene oxide–gold nanocomposites modified electrode for amperometric detection of organophosphorus and carbamate pesticidesSensors and Actuators B: Chemical, 2011
- Amplified immunosensing based on ionic liquid-doped chitosan film as a matrix and Au nanoparticle decorated graphene nanosheets as labelsElectrochimica Acta, 2011
- A highly sensitive and rapid organophosphate biosensor based on enhancement of CdS–decorated graphene nanocompositeAnalytica Chimica Acta, 2011
- Electrochemical SensorsAnalytical Chemistry, 2008
- Changes of pesticide residues in apples during cold storageFood Control, 2008
- Evaluation of 10 pesticide residues in oranges and tangerines from Valencia (Spain)Food Control, 2006
- Real-time detection of chlorpyrifos at part per trillion levels in ground, surface and drinking water samples by a portable surface plasmon resonance immunosensorAnalytica Chimica Acta, 2006
- Automated detection of chlorpyrifos and its metabolites by a continuous flow system-based enzyme sensorAnalytica Chimica Acta, 2001
- Preparation of Graphitic OxideJournal of the American Chemical Society, 1958