Hormonally regulated phosphoprotein of turkey erythrocytes: localization to plasma membrane.

Abstract

The catecholamine-stimulated cotransport of Na+ and K+ across the plasma membrane of the turkey erythrocyte was previously found to be associated with increased 32P incorporation into a high MW protein. To determine the subcellular localization of this phosphorylated protein, which was termed goblin, a new method was developed for isolation of pure plasma membranes from turkey erythrocytes. With this method, goblin was located in the plasma membrane. Goblin was not extracted by solutions of low or high ionic strength but was partially extracted by nonionic detergents, indicating that it is not a component of turkey erythrocyte spectrin and suggesting that it may be an intrinsic protein of the plasma membrane. The data are compatible with a possible role for goblin in the hormonal control of ion movements across the plasma membrane.