Evidence for Horizontal Gene Transfer of Two Antigenically Distinct O Antigens in Bordetella bronchiseptica
- 1 August 2009
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 77 (8), 3249-3257
- https://doi.org/10.1128/iai.01448-08
Abstract
Host immunity is a major driving force of antigenic diversity, resulting in pathogens that can evade immunity induced by closely related strains. Here we show that two Bordetella bronchiseptica strains, RB50 and 1289, express two antigenically distinct O-antigen serotypes (O1 and O2, respectively). When 18 additional B. bronchiseptica strains were serotyped, all were found to express either the O1 or O2 serotype. Comparative genomic hybridization and PCR screening showed that the expression of either the O1 or O2 serotype correlated with the strain containing either the classical or alternative O-antigen locus, respectively. Multilocus sequence typing analysis of 49 B. bronchiseptica strains was used to build a phylogenetic tree, which revealed that the two O-antigen loci did not associate with a particular lineage, evidence that these loci are horizontally transferred between B. bronchiseptica strains. From experiments using mice vaccinated with purified lipopolysaccharide from strain RB50 (O1), 1289 (O2), or RB50Δ wbm (O antigen deficient), our data indicate that these O antigens do not confer cross-protection in vivo. The lack of cross-immunity between O-antigen serotypes appears to contribute to inefficient antibody-mediated clearance between strains. Together, these data are consistent with the idea that the O-antigen loci of B. bronchiseptica are horizontally transferred between strains and encode antigenically distinct serotypes, resulting in inefficient cross-immunity.Keywords
This publication has 43 references indexed in Scilit:
- Role of the Type III Secretion System in a Hypervirulent Lineage of Bordetella bronchisepticaInfection and Immunity, 2009
- Replacement of Adenylate Cyclase Toxin in a Lineage of Bordetella bronchisepticaJournal of Bacteriology, 2008
- Saccharide/protein conjugate vaccines for Bordetella species: Preparation of saccharide, development of new conjugation procedures, and physico-chemical and immunological characterization of the conjugatesVaccine, 2008
- The O Antigen Enables Bordetella parapertussis To Avoid Bordetella pertussis -Induced ImmunityInfection and Immunity, 2007
- Comparative Role of Immunoglobulin A in Protective Immunity against the BordetellaeInfection and Immunity, 2007
- Construction and validation of a first-generation Bordetella bronchiseptica long-oligonucleotide microarray by transcriptional profiling the Bvg regulonBMC Genomics, 2007
- Serotype cycles in cholera dynamicsProceedings Of The Royal Society B-Biological Sciences, 2006
- Bordetella pertussis, the Causative Agent of Whooping Cough, Evolved from a Distinct, Human-Associated Lineage of B. bronchisepticaPLoS Pathogens, 2005
- Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspeciesClinical Microbiology Reviews, 2005
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970