Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy.
Open Access
- 7 November 1995
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 92 (23), 10648-10652
- https://doi.org/10.1073/pnas.92.23.10648
Abstract
The structures of Sindbis virus and Ross River virus complexed with Fab fragments from monoclonal antibodies have been determined from cryoelectron micrographs. Both antibodies chosen for this study bind to regions of the virions that have been implicated in cell-receptor recognition and recognize epitopes on the E2 glycoprotein. The two structures show that the Fab fragments bind to the outermost tip of the trimeric envelope spike protein. Hence, the same region of both the Sindbis virus and Ross River virus envelope spike is composed of E2 and is involved in recognition of the cellular receptor.Keywords
This publication has 41 references indexed in Scilit:
- Low pH induces swiveling of the glycoprotein heterodimers in the Semliki forest virus spike complexCell, 1995
- Structure Determination of an Fab Fragment that Neutralizes Human Rhinovirus 14 and Analysis of the Fab-Virus ComplexJournal of Molecular Biology, 1994
- The Organization of the Spike Complex of Semliki Forest VirusJournal of Molecular Biology, 1994
- Three-dimensional structure of a membrane-containing virus.Proceedings of the National Academy of Sciences of the United States of America, 1993
- Refined Structure of Sindbis Virus Core Protein and Comparison with Other Chymotrypsin-like Serine Proteinase StructuresJournal of Molecular Biology, 1993
- Crystallization of Sindbis virus and its nucleocapsidJournal of Molecular Biology, 1992
- Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the organization of the virionNature, 1991
- Topological organization of Sindbis virus capsid protein in isolated nucleocapsidsVirus Research, 1987
- Envelope structure of Semliki Forest virus reconstructed from cryo-electron micrographsNature, 1986
- Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971