Developmental and molecular correlates of bovine preimplantation embryos
- 1 May 2006
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 131 (5), 895-904
- https://doi.org/10.1530/rep.1.01021
Abstract
Expression of embryonic genes is altered in different culture conditions, which influence developmental potential both during preimplantation and fetal development. The objective of this study was to define the effects of culture conditions on: bovine embryonic development to blastocyst stage, blastocyst cell number, apoptosis and expression patterns of a panel of developmentally important genes. Bovine embryos were culturedin vitroin three culture media containing amino acids, namely potassium simplex optimization medium (KSOMaa), Charles Rosenkrans 1 (CR1aa) and synthetic oviductal fluid (SOFaa). Apoptosis in blastocysts was determined by TUNEL assay and expression profiles of developmentally important genes were assayed by real-time PCR.In vivo-produced bovine blastocysts were used as controls for experiments determining gene expression patterns. While the cleavage rates did not differ, embryos cultured in SOFaa had higher rates of development to blastocyst stage (P< 0.05). Mean cell numbers and percentages of apoptotic cells per blastocyst did not differ among the groups. Expression of the heat shock protein 70 (Hsp70) gene was significantly up-regulated in both CR1aa and KSOMaa when compared with SOFaa (P< 0.001). DNA methyltransferase 3a (Dnmt3a) expression was higher in embryos cultured in CR1aa than in those cultured in SOFaa (P< 0.001). Expression of interferon tau (IF-τ) and insulin-like growth factor II receptor (Igf-2r) genes was significantly up-regulated in KSOMaa when compared with CR1aa (P< 0.001). Gene expression did not differ betweenin vivo-derived blastocysts and theirin vitro-derived counterparts. In conclusion, SOFaa supports higher development to blastocyst stage than KSOMaa and CR1aa, and the culture conditions influence gene expression.Keywords
This publication has 40 references indexed in Scilit:
- A Comparative Expression Analysis of Gene Transcripts in Post‐fertilization Developmental Stages of Bovine Embryos Produced in Vitro or in VivoReproduction in Domestic Animals, 2004
- Temporal Divergence in the Pattern of Messenger RNA Expression in Bovine Embryos Cultured from the Zygote to Blastocyst Stage In Vitro or In VivoBiology of Reproduction, 2003
- Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCRNucleic Acids Research, 2002
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Embryonic genome activationFrontiers in Bioscience-Landmark, 2001
- Expression of Growth Factor Ligand and Their Receptor mRNAs in Bovine Ova during In Vitro Maturation and after Fertilization In Vitro.The Journal of Veterinary Medical Science, 1998
- Effects of Glutamine and Taurine on Preimplantation Development and Cleavage of Mouse Embryos in Vitro1Biology of Reproduction, 1997
- Elevated Temperature Increases Heat Shock Protein 70 Synthesis in Bovine Two-Cell Embryos and Compromises Function of Maturing Oocytes1Biology of Reproduction, 1996
- Mouse preimplantation embryo development in vitro: Effectof sodium concentration in culture media on RNA synthesis and accumulation and gene expressionMolecular Reproduction and Development, 1994
- Effects of epinephrine and hypotaurine on in-vitro fertilization in the golden hamsterReproduction, 1982