Quantitation of γ-trace in human biological fluids: Indications for production in the central nervous system
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis Ltd in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 39 (7), 619-626
- https://doi.org/10.3109/00365517909108866
Abstract
Gamma-Trace was purified in large amounts from urine and used for the production of a specific rabbit antiserum. An enzyme immunoassay for quantitation of gamma-trace was developed using the pure protein as a primary standard. Its sensitivity was approximately 30 microgram/l. An enzyme amplified single radial immunodiffusion was developed as well. Its sensitivity was approximately 0.3 mg/l. These assays allowed quantitation of gamma-trace in normal human biological fluids. The following results were obtained (mean +/- SD): cerebrospinal fluid: 5.8 +/- 2.2 mg/l, plasma: 1.1 +/- 0.42 mg/l, saliva: 1.8 +/- 0.88 mg/l and urine: 0.095 +/- 0.057 mg/l. Plasma samples from patients with advanced renal failure revealed gamma-trace values up to 13 times the normal mean plasma value. The results indicate a production of gamma-trace in the central nervous system and that the protein is primarily catabolized by the kidney.Keywords
This publication has 20 references indexed in Scilit:
- A heated biuret-Folin protein assay which gives equal absorbance with different proteinsAnalytical Biochemistry, 1977
- Occurrence of β2-microglobulin and post-γ globulin in human semenClinica Chimica Acta; International Journal of Clinical Chemistry, 1976
- Post-γ-globulin: Isolation and physicochemical characterizationArchives of Biochemistry and Biophysics, 1973
- Crossed ImmunoelectrophoresisScandinavian Journal of Clinical and Laboratory Investigation, 1972
- Coupling of enzymes to proteins with glutaraldehyde: Use of the conjugates for the detection of antigens and antibodiesImmunochemistry, 1969
- Trace Proteins in Biological Fluids. IV. Physicochemical Properties and Sites of Formation of -Trace and -Trace Proteins.Experimental Biology and Medicine, 1967
- Trace proteins in cerebrospinal fluid and other biological fluids I. Effect of various fractionation procedures on β-trace and γ-trace proteins and methods for isolation of both proteinsArchives of Biochemistry and Biophysics, 1963
- Use of an Antiserum Against Cerebrospinal Fluid in Demonstration of Trace Proteins in Biological Fluids.Experimental Biology and Medicine, 1962
- The occurrence of post-gamma protein in urine: a new protein abnormalityJournal of Clinical Pathology, 1961
- The True Endogenous Creatinine ClearanceScandinavian Journal of Clinical and Laboratory Investigation, 1953