SEGMENTAL RESPONSE OF THE MACROPHAGE PLASMA MEMBRANE TO A PHAGOCYTIC STIMULUS
Open Access
- 1 February 1974
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 139 (2), 323-336
- https://doi.org/10.1084/jem.139.2.323
Abstract
A method of attaching mouse RBCs to mouse macrophages is described. Both cell types were coated with rabbit anti-mouse macrophage F(ab')(2), and cross-linkage of cells was effected with sheep F(ab')(2) directed against rabbit F(ab')(2). 98% of macrophages attached an average of 11 RBCs each. Attachment occurred at 37 degrees C and was stable for at least 4 h. Less than 0.1% of macrophages ingested RBCs under these conditions. Latex particles and opsonized pneumococci were ingested as avidly by RBC-coated macrophages as by native macrophages. Ingestion of these particles did not prompt ingestion of attached RBCs. When anti-RBC IgG was added, however, over 90% of macrophages ingested an average of six RBCs each. Thus, ingestion of one particle does not trigger generalized phagocytosis of all particles attached to the cell's plasma membrane, and the phagocytic stimulus is confined to the segment of the cell's plasma membrane immediately adjacent to the particle being ingested.Keywords
This publication has 21 references indexed in Scilit:
- Action of concanavalin A on the attachment stage of phagocytosis by macrophagesExperimental Cell Research, 1971
- EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTESThe Journal of Experimental Medicine, 1971
- MACROPHAGE PLASMA MEMBRANESThe Journal of Experimental Medicine, 1971
- THE INTERACTION IN VITRO OF MYCOPLASMA PULMONIS WITH MOUSE PERITONEAL MACROPHAGES AND L-CELLSThe Journal of Experimental Medicine, 1971
- Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.1970
- ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATEThe Journal of cell biology, 1968
- Receptor Sites of Human Monocytes for IgGInternational Archives of Allergy and Immunology, 1968
- Studies on the Immunoglobulins Which Stimulate the Ingestion of Glutaraldehyde-Treated Red Cells Attached to MacrophagesThe Journal of Immunology, 1967
- The dissociation of the attachment and ingestion phases of phagocytosis by macrophagesExperimental Cell Research, 1967
- EFFECTS OF ANTICELLULAR SERUM ON PHAGOCYTOSIS AND THE UPTAKE OF TRITIATED THYMIDINE AND URIDINE BY HELA CELLSThe Journal of Experimental Medicine, 1965