Supplementation of Methionine Enhanced the Ergothioneine Accumulation in the Ganoderma neo-japonicum Mycelia
- 8 August 2008
- journal article
- Published by Springer Science and Business Media LLC in Applied Biochemistry and Biotechnology
- Vol. 158 (1), 213-221
- https://doi.org/10.1007/s12010-008-8322-0
Abstract
The methods for increasing the production of ergothioneine (ERG) were investigated by using the mycelial culture of several mushroom species, primarily Ganoderma neo-japonicum. We first found that ERG was accumulated at the different levels in mycelia and fruiting bodies, respectively, depending on the mushroom species. As a result of adding various amino acids to the mycelial culture medium, methionine (Met) was shown to be the most effective additive. The most preferable condition of the additive was the combination of 4 mM Met and 1 g/l of yeast extract, and the maximum ERG production reached approximately 1.7 mg/l, which corresponds to 2.4 times (0.7 mg/l) that in the basal medium without Met. Although the supplementation of Met enhanced the ERG production, the mycelial growth was significantly inhibited. Furthermore, the analysis of amino acids in the culture medium revealed that the Met additive reduced the consumption rates of most amino acids tested, probably due to the decrease in mycelial growth. Taking these results into consideration, we suggest that the addition of Met to the mycelial culture medium is an efficient way to enhance the ERG production in economically important mushroom species.Keywords
This publication has 23 references indexed in Scilit:
- Factors influencing the production of endopolysaccharide and exopolysaccharide from Ganoderma applanatumEnzyme and Microbial Technology, 2007
- Identification and Quantification of Ergothioneine in Cultivated Mushrooms by Liquid Chromatography-Mass SpectroscopyInternational Journal of Medicinal Mushrooms, 2006
- Ergothioneine rescues PC12 cells from β-amyloid-induced apoptotic deathFree Radical Biology & Medicine, 2004
- Antioxidant activity and total phenolics of edible mushroom extractsFood Chemistry, 2003
- Antioxidant properties of several specialty mushroomsFood Research International, 2002
- Antioxidant properties of several commercial mushroomsFood Chemistry, 2002
- ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF EDIBLE MUSHROOMSJournal of Food Lipids, 2002
- Antioxidant Action of Ergothioneine: Assessment of Its Ability to Scavenge PeroxynitriteBiochemical and Biophysical Research Communications, 1997
- Interception of some direct‐acting mutagens by ergothioneineEnvironmental and Molecular Mutagenesis, 1987
- Quantitative determination of ergothioneine in plasma and tissues by TLC-densitometry.CHEMICAL & PHARMACEUTICAL BULLETIN, 1980