A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
Open Access
- 17 January 2005
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 168 (2), 179-184
- https://doi.org/10.1083/jcb.200409053
Abstract
Nucleostemin (NS) was identified as a stem cell– and cancer cell–enriched nucleolar protein that controls the proliferation of these cells. Here, we report the mechanism that regulates its dynamic shuttling between the nucleolus and nucleoplasm. The nucleolar residence of nucleostemin involves a transient and a long-term binding by the basic and GTP-binding domains, and a dissociation mechanism mediated by the COOH-terminal region. This cycle is propelled by the GTP binding state of nucleostemin. We propose that a rapid nucleostemin cycle is designed to translate extra- and intra-cellular signals into the amount of nucleostemin in the nucleolus in a bidirectional and fast manner.Keywords
This publication has 22 references indexed in Scilit:
- Guarding the guardian?Nature, 2004
- The Putative GTPases Nog1p and Lsg1p Are Required for 60S Ribosomal Subunit Biogenesis and Are Localized to the Nucleus and Cytoplasm, RespectivelyMolecular and Cellular Biology, 2003
- A nucleolar mechanism controlling cell proliferation in stem cells and cancer cellsGenes & Development, 2002
- Nucleolomics: An Inventory of the NucleolusMolecular Cell, 2002
- Paraspeckles: A Novel Nuclear DomainCurrent Biology, 2002
- Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase CellsThe Journal of cell biology, 2001
- Nucleolar size indicates the rapidity of cell proliferation in cancer tissuesThe Journal of Pathology, 2000
- Non-activated p53 co-localizes with sites of transcription within both the nucleoplasm and the nucleolusOncogene, 2000
- IL‐3 and ribavirin induce differentiation and growth suppression during long‐term treatment of a megakaryocytic leukemia cell lineJournal of Cellular Physiology, 1995
- Induction of erythroid differentiation and modulation of gene expression by tiazofurin in K-562 leukemia cells.Proceedings of the National Academy of Sciences of the United States of America, 1988