Functional multiplex reporter assay using tagged Gaussia luciferase
Open Access
- 10 January 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), srep01046
- https://doi.org/10.1038/srep01046
Abstract
We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluctag), each expressed in different tumor cells. By immunobinding of the tags followed by Gluctag detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.This publication has 18 references indexed in Scilit:
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