Separation and quantification of ropinirole and some impurities using capillary liquid chromatography
- 24 September 1999
- journal article
- Published by Elsevier BV in Journal of Chromatography B: Biomedical Sciences and Applications
- Vol. 732 (2), 437-444
- https://doi.org/10.1016/s0378-4347(99)00314-x
Abstract
Ropinirole, 4-[2-(dipropylamino)ethyl]-1,3-dihydro-2H-indol-2-one, is a potent anti-Parkinson’s disease drug developed by SmithKline Beecham Pharmaceuticals. Capillary liquid chromatography (CLC) was used for the separation and quantification of ropinirole and its five related impurities, potentially formed during its synthesis. A simultaneous optimization of three mobile phase parameters, i.e., pH, buffer concentration and acetonitrile content was performed employing an experimental design approach which proved a powerful tool in method development. The retention factors of the investigated substances in different mobile phases were determined. Baseline resolution of the six substances on a C18 reversed stationary phase was attained using a mobile phase with an optimized composition [acetonitrile–8.7 mM 2-(N-morpholino)ethanesulfonic acid adjusted to pH 6.0 (55:45, v/v)]. It was shown that CLC, operated in the isocratic mode under the mobile phase flow-rate of 4 μl/min, can determine the level of these impurities, down to a level of 0.06% of the main component within 25 min.Keywords
This publication has 18 references indexed in Scilit:
- High-Sensitivity Detection of 4-(3-Pyridinylmethylamino-carboxypropyl) Phenylthiohydantoins by Capillary Liquid Chromatography–Microelectrospray Ion Trap Mass SpectrometryProtein Journal, 1997
- An affordable high-performance optical absorbance detector for capillary systemsAnalytica Chimica Acta, 1997
- Determination of Acaricides in Honey by Liquid Chromatography with Ordinary, Narrow-Bore, and Microbore ColumnsAnalytical Chemistry, 1996
- Tutorial review. Future prospects for the analysis of complex biological systems using micro-column liquid chromatography–electrospray tandem mass spectrometryThe Analyst, 1996
- Development of an ion trap / linear time‐of‐flight mass spectrometer with electrospray ionization for micro‐column liquid chromatography detectionJournal of Microcolumn Separations, 1995
- Micro high-performance liquid chromatography for the determination of nicarbazin in chicken tissues, eggs, poultry feed and litterJournal of Chromatography A, 1995
- Analysis of coumarins by micro high-performance liquid chromatography-mass spectrometry with a particle beam interfaceJournal of the American Society for Mass Spectrometry, 1995
- Electrochromatography and micro high-performance liquid chromatography with 320 μm I.D. packed columnsJournal of Chromatography A, 1994
- Rapid analysis of ribonucleosides and bases at the picomole level using pellicular cation exchange resin in narrow bore columnsAnalytical Chemistry, 1969
- Fast liquid chromatography. Investigation of operating parameters and the separation of nucleotides on pellicular ion exchangersAnalytical Chemistry, 1967