Activation of the protein kinase C-mediated contractile system in canine basilar artery undergoing chronic vasospasm.
- 1 September 1991
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Stroke
- Vol. 22 (9), 1183-1187
- https://doi.org/10.1161/01.str.22.9.1183
Abstract
We previously suggested that activation of the protein kinase C-mediated contractile system may participate in the occurrence of chronic cerebral vasospasm. In the present study, we compared segments of normal beagle basilar arteries in vitro with segments of arteries undergoing chronic vasospasm to determine the responsiveness to various agonists such as serotonin, prostaglandin F2 alpha, and phorbol 12,13-diacetate as well as to external Ca2+. We also compared the effects of W-7 (a calmodulin inhibitor), nicardipine (a calcium channel blocker), and H-7 (a protein kinase C inhibitor) on the spontaneous tonus of arterial segments stabilized at a resting tension of 3 g. Compared with normal segments, the responsiveness to each agonist in segments undergoing vasospasm was essentially unchanged whereas the the responsiveness to external Ca2+ was significantly decreased (p less than 0.001). In segments undergoing vasospasm the decrease in resting tension induced by W-7 was markedly diminished (p less than 0.01), that induced by nicardipine was unchanged, and that induced by H-7 was significantly increased (p less than 0.01). Our results indicate that spontaneous tonus due to activation of the protein kinase C system is significantly augmented in segments undergoing vasospasm. Thus this system, rather than the Ca2+/calmodulin system, appears to play a major role in the occurrence of chronic vasospasm.Keywords
This publication has 15 references indexed in Scilit:
- Possible Role of Protein Kinase C-Dependent Smooth Muscle Contraction in the Pathogenesis of Chronic Cerebral VasospasmJournal of Cerebral Blood Flow & Metabolism, 1991
- Phorbol 12,13-Diacetate-Induced Contraction of the Canine Basilar Artery: Role of Protein Kinase CJournal of Cerebral Blood Flow & Metabolism, 1991
- Impairment of Vascular Reactivity and Changes in Intracellular Calcium and Calmodulin Levels of Smooth Muscle Cells in Canine Basilar Arteries after Subarachnoid HemorrhageNeurosurgery, 1989
- Alterations of mechanical properties in canine basilar arteries after subarachnoid hemorrhageJournal of Neurosurgery, 1989
- Arterial Wall Changes in Chronic Cerebrovasospasm: In Vitro and In Vivo Pharmacological EvidenceAnnual Review of Pharmacology and Toxicology, 1988
- Intrathecal Nimodipine Therapy in a Primate Model of Chronic Cerebral VasospasmNeurosurgery, 1988
- Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase CBiochemistry, 1984
- A randomized placebo-controlled double-blind trial of nimodipine after SAH in monkeysJournal of Neurosurgery, 1984
- R 24571: A new powerful inhibitor of red blood cell Ca++-transport ATPase and of calmodulin-regulated functionsBiochemical and Biophysical Research Communications, 1981
- Structural changes of the intradural arteries following subarachnoid hemorrhageJournal of Neurosurgery, 1972