Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR
Open Access
- 1 March 2003
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (3), 1844-1846
- https://doi.org/10.1128/aem.69.3.1844-1846.2003
Abstract
In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.Keywords
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