Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory
Top Cited Papers
Open Access
- 19 September 2012
- journal article
- Published by Springer Science and Business Media LLC in BMC Bioinformatics
- Vol. 13 (1), 238
- https://doi.org/10.1186/1471-2105-13-238
Abstract
Recent methods have been developed to perform high-throughput sequencing of DNA by Single Molecule Sequencing (SMS). While Next-Generation sequencing methods may produce reads up to several hundred bases long, SMS sequencing produces reads up to tens of kilobases long. Existing alignment methods are either too inefficient for high-throughput datasets, or not sensitive enough to align SMS reads, which have a higher error rate than Next-Generation sequencing.Keywords
This publication has 35 references indexed in Scilit:
- Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precisionNature Biotechnology, 2012
- Origins of theE. coliStrain Causing an Outbreak of Hemolytic–Uremic Syndrome in GermanyThe New England Journal of Medicine, 2011
- Graphene as a subnanometre trans-electrode membraneNature, 2010
- Direct detection of DNA methylation during single-molecule, real-time sequencingNature Methods, 2010
- Fast and accurate long-read alignment with Burrows–Wheeler transformBioinformatics, 2010
- Personalized copy number and segmental duplication maps using next-generation sequencingNature Genetics, 2009
- Fast and accurate short read alignment with Burrows–Wheeler transformBioinformatics, 2009
- SOAP: short oligonucleotide alignment programBioinformatics, 2008
- BLAT—The BLAST-Like Alignment ToolGenome Research, 2002
- A Greedy Algorithm for Aligning DNA SequencesJournal of Computational Biology, 2000