The Influence of Alendronate on Spine Fusion in an Osteoporotic Animal Model
- 1 August 2011
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Spine
- Vol. 36 (18), 1446-1452
- https://doi.org/10.1097/brs.0b013e3181f49c47
Abstract
A posterolateral lumbar fusion model in osteoporotic rats. OBJECTIVE.: To assess the effect of alendronate on spine fusion in an osteoporotic animal model. The effect of alendronate on spine fusion is still controversial. Also, there are no studies using an osteoporotic animal model to assess the effect of alendronate on spine fusion. Forty-six female Sprague-Dawley rats underwent either sham-operation (sham) (N = 24) or bilateral ovariectomy (OVX)(N = 22). Eight weeks after the first surgery, animals underwent intertransverse spine fusion at L4-L5. Animals received saline or alendronate 70 μg/kg/wk by subcutaneous administration once a week for 8 weeks after spinal arthrodesis. All animals were divided into four groups: sham-control, sham-alendronate, OVX-control, and OVX-alendronate. After that the animals were killed and the fusion mass was assessed by radiographic, peripheral quantitative computed tomography (pQCT) scanning, and biomechanical and histologic analysis. In the radiographic study and the pQCT scanning, the area of fusion masses of animals treated with alendronate was considerably larger and denser than that of the control animals in both sham and OVX groups. In the biomechanical study, the ultimate load of the fusion mass of alendronate-treated animals was higher than that of control animals in the osteoporotic groups. Histologic analysis of sagittal sections of fusion mass revealed greater new bone formation in alendronate-treated animals in osteoporotic models and significant inhibition of osteoclasts among the grafted area of alendronate-treated animals compared with the control models. Alendronate was effective for radiologic, biomechanical, and histologic success of spine fusion in an osteoporotic animal model. Though much of the graft bone was not resorbed, alendronate increased biomechanical strength with ingrowth of new bone formation in osteoporotic animals. This study suggests the alendronate may improve spine fusion healing in the presence of osteoporosis.Keywords
This publication has 24 references indexed in Scilit:
- Effect of local delivery of alendronate on bone formation in bioactive glass grafting in ratsOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2007
- Alendronate increases BMD at appendicular and axial skeletons in patients with established osteoporosisJournal of Orthopaedic Surgery and Research, 2007
- Alendronate Inhibits Spine Fusion in a Rat ModelSpine, 2005
- The Influence of Alendronate Treatment and Bone Graft Volume on Posterior Lateral Spine Fusion in a Porcine ModelSpine, 2005
- High-dose Alendronate Uncouples Osteoclast and Osteoblast FunctionClinical Orthopaedics and Related Research, 2004
- The effect of alendronate sodium on spinal fusion: a rabbit modelThe Spine Journal, 2004
- Alendronate mechanism of action: geranylgeraniol, an intermediate in the mevalonate pathway, prevents inhibition of osteoclast formation, bone resorption, and kinase activation in vitroProceedings of the National Academy of Sciences of the United States of America, 1999
- Effect of Alendronate on Risk of Fracture in Women With Low Bone Density but Without Vertebral FracturesResults From the Fracture Intervention TrialJAMA, 1998
- Bisphosphonate action. Alendronate localization in rat bone and effects on osteoclast ultrastructure.JCI Insight, 1991
- Effects of bisphosphonates on isolated Rat osteoclasts as examined by reflected light microscopyJournal of Bone and Mineral Research, 1990